黄颡鱼(Pelteobagrus fulvidraco)“红头病”病原菌迟钝爱德华氏菌(Edwardsiella tarda)的分离及鉴定  

ISOLATION AND IDENTIFICATION OF BACTERIOSIS PATHOGEN——EDWARDSIELLA TARDA FROM YELLOW CARTFISH(PELTEOBAGRUS FULVIDRACO) WITH RED HEAD DISEASE

作　　者：邓先余[1,2] 罗文[2,3] 谭树华[1] 邱山红 陈康贵[1] 

机构地区：[1]湖南科技大学生命科学学院,湘潭411201 [2]浙江大学动物科技学院,杭州310058 [3]绍兴文理学院生命科学学院,绍兴312000 [4]湖南省怀化市第三中学,怀化418000

出　　处：《海洋与湖沼》2008年第5期511-516,共6页Oceanologia Et Limnologia Sinica

基　　金：湖南省教育厅资助项目,B30512号;湖南科技大学博士基金资助项目,E50437号

摘　　要：用ATB微生物自动鉴定系统对分离自湖南湘潭地区人工养殖的患"红头病"的黄颡鱼体内的2株细菌(即HN004和HN005)进行了鉴定,发现它们的生理生化特征完全相同,均为革兰氏阴性短杆菌、接触酶阳性、吲哚阳性、H2S阳性;氧化酶阴性、V.P测定为阴性,与迟钝爱德华氏菌的表型特征非常相似。为进一步确定2株菌的分类学地位,测定了其16SrRNA基因序列,分析了相关细菌相应序列的同源性,构建分子系统发育树。结果表明,2菌株的序列完全一致,与迟钝爱德华氏菌的亲缘关系最近,相似性为99.0%。综合上述结果,2菌株可鉴定为迟钝爱德华氏菌(Edwardsiella tarda)。Using ATB Microbiology Automatic Identification System, two bacterial strain HN004 and HN005 isolated from cultured yellow cartfish （Pelteobagrus fulvidraco） infected with typical ＂Red head Disease＂ were identified. The two strains had completely the same physiological and biochemical characteristics： Gram-negative, rod-shaped, catalase-positive, indole-posifive, H2S-positive, oxidase-negative, and V.P-negative; and were very similar to Edwardsiella tarda in most of the phenotypes. In order to determine the phylogenetic position of these two microorganism, their 16S rRNA genes were sequenced and compared with those of related strains. The molecular phylogenetic dendrogram was constructed based on the genetic distance analysis, which revealed that the 16S rDNA sequences of strain HN004 and HN005 were completely the same, and they exhibited the highest levels of similarity （99.0%） to E. tarda. Therefore, strains HN004 and HN005 were identified as E. tarda.

关 键 词：黄颡鱼 红头病 迟钝爱德华氏菌 分离 鉴定 

分 类 号：S942[农业科学—水产养殖]