曲马多对神经病理性疼痛大鼠P2X3受体表达的影响  

Effect of tramadol on P2X_3 receptor expression in rats with chronic constriction injury

作　　者：刘雪[1] 鄢建勤[2] 王世端[1] 

机构地区：[1]青岛大学医学院附属医院麻醉科,青岛市266003 [2]中南大学湘雅医院麻醉科

出　　处：《临床麻醉学杂志》2008年第9期782-784,共3页Journal of Clinical Anesthesiology

摘　　要：目的研究曲马多对大鼠神经病理性疼痛的镇痛作用及背根神经节(DRG)P2X_3受体表达的影响。方法 30只 SD 大鼠建立慢性缩窄性损伤(CCI)模型后随机分为假手术组(S 组,不给药)和手术组(T5组、T10组、T20组、NS 组),于术后第3天开始腹腔分别注射不同剂量的曲马多(5、10、20 mg/kg)及生理盐水,各组大鼠分别于术前和术后第3、7、10、14天测定大鼠右后足热痛阈,术后第14天取大鼠 DRG 采用免疫组化法测定 P2X_3。结果术后大鼠右后足热痛阈明显缩短。P2X_3在 NS 组表达最高,NS、T5、T10组明显高于 S 组(P<0.05);T20组表达最低,与 S 组相比差异无统计学意义。结论曲马多能剂量依赖性地缓解 CCI 大鼠的热痛觉过敏现象,达到镇痛效果,同时能使 P2X_3表达减少。Objective To study the analgesic effect of tramadol in rat with chronic constriction injury（CCI） and the expression of P2X3 receptor. Methods Thirty SD rats were randomly divided into five groups of control group, group T5 （ tramadol 5 mg/kg）, T10 （tramadol 10 mg/kg）, T20 （tramadol 20 mg/kg）, and sham-operated group （S group）. Hyperalgesia was measured before operation and on the 3^rd , 7^th , 10^th , 14^th postoperative day. Three days after CCI, different doses of tramadol were administered. The P2X3 receptor expression was determined in dorsal root ganglion by immunohistochemistry method on the 14^th. Results After CCI, all rats developed hyperalgesia, and tramadol relieved dose-dependently it . The expressin of P2X3 receptor was reduced in the rats given tramadol. Conclusion Tramadol could attenuate hyperalgesia of CCI rats,and reduce the expression of P2X3 receptor.

关 键 词：曲马多 神经病理性疼痛 痛觉过敏 慢性缩窄性损伤 嘌呤P2受体 

分 类 号：R741[医药卫生—神经病学与精神病学]