流动注射化学发光法测定青霉胺  

FI-Chemiluminescence Determination of Penicillamine

作　　者：李利军[1] 钟招亨[2] 陈其锋[2] 冯军[2] 孔红星[1] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]广西大学化学与化学工程学院,南宁530004

出　　处：《理化检验（化学分册）》2008年第9期868-870,877,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广西科学基金资助(0640029)

摘　　要：在酸性条件下高碘酸钠氧化过氧化氢产生弱发光,青霉胺的加入能大大增强此弱发光,据此,建立了流动注射化学发光法测定青霉胺的方法。该方法的线性范围为1.O×10^(-6)～8.0×10^(-4)mol·L^(-1),检出限为5.6×10^(-7)mol·L^(-1),对8.0×10^(-5)mol·L^(-1)的青霉胺进行7次平行测定,其相对标准偏差为1.9%。已应用于青霉胺片中青霉胺含量的测定,结果与中国药典法测得值一致。在青霉胺片中加入6×10^(-5)～2×10^(-4)mol·L^(-1)青霉胺标准进行回收试验,测得回收率在97.8%～104.4%之间。对化学发光反应的化学机理也作了简要探讨。The weak chemiluminescence （CL）, produced by the redox reaction between sodium periodate and hydrogen peroxide in dil. H3PO4 medium, was greatly enhanced by the presence of penicillamine. Base on this fact, and by applying the flow injection technique, a new method of FI-CL determination of penicillamine was praposed. Linear relationship between CL intensity and concentration of pencillamine was obtained in the range of 1.0× 10^-6 ～ 8. 0×10^-4mol · L^-1, with a detection limit of 5. 6× 10^-7mol · L^-1. Precision of the method was tested at the concentration level of 8. 0 × 10^-5 mol · L^-1 of penicillamine with 7 determinations, giving value of RSD of 1.9 %. In the determination of penicillamine content in a sample of penicillamine tablets, the result obtained was in consistency with the result obtained by pharmacopeia method. Test for recovery was made by adding standard penicillamine solution in different amounts from 6 ×10^-5 to 2 × 10^-4 mol · L^-1, and values of recovery obtained were in the range of 97. 8% to 104.4%. A brief discussion of chemism of the CL reaction was also given.

关 键 词：化学发光测定 流动注射 高碘酸钠 过氧化氢 青霉胺 

分 类 号：O657.3[理学—分析化学]