bcl-X_L在急性髓系白血病细胞的表达及与化疗敏感性的关系  

Relationship between bclXL expression and sensitivity to chemotherapy in acute myeloid leukemic cells

作　　者：周剑锋[1,2,3] 陈燕[1,2,3] 李崇渔[1,2,3] 刘文励 杨林花[1,2,3] 乔振华 周木想[1,2,3] 王辨明 

机构地区：[1]同济医科大学附属协和医院血液科 [2]同济医科大学附属同济医院血液科 [3]山西医科大学第二附属医院血液科

出　　处：《中华血液学杂志》1997年第11期584-587,共4页Chinese Journal of Hematology

基　　金：国家教委留学回国人员课题

摘　　要：目的：探讨急性髓系白血病（ＡＭＬ）细胞凋亡径路的特征及调控模式。方法：运用流式细胞仪（ＦＡＣＳ）、ＤＮＡ电泳、Ｎｏｒｔｈｅｒｎｂｌｏｔ分析技术，观察了化疗药物足叶乙甙（Ｖｐ１６）作用于原代ＡＭＬ细胞前后，ｂｃｌ－ＸＬ表达与凋亡的敏感性、临床特征、预后的关系。结果：绝大部分ＡＭＬ高表达ｂｃｌ－ＸＬｍＲＮＡ病例经Ｖｐ１６处理后，其ｂｃｌ－ＸＬ虽然下调，但仍维持较高水平，并对凋亡诱导不敏感，与高白细胞数、髓外浸润等不良预后特征相关，化疗后不易缓解，处于长期缓解中的病例经Ｖｐ１６处理后，ｂｃｌ－ＸＬ亦表达下调，但凋亡率远低于ＡＭＬ细胞。结论：ｂｃｌ－ＸＬ高表达与ＡＭＬ的发生、维持和化疗耐药性相关，ｂｃｌ－ＸＬ高表达下调是凋亡启动的基础，但本身不足以引致凋亡。Objective:To investigate the characteristics and regulation of apoptosis in acute myeloid leukemic cells.Methods:Flow cytometry,DNA electrophoresis and Northern blot were used to analyze the expression of bclXL before and after Vp16 treatment,and the relationships between bclXL expression and apoptotic sensitivity, clinical features and prognosis were also analyzed.Results:Most of the leukemic cells from AML patients overexpressed bclXL mRNA.The expression of bclXL was still maintained high level after Vp16 treatment in patients with pretreatment overexpression.These patients were resistant to apoptosis,poor responsed to chemotherapy and associated with adverse prognostic features,such as high WBC counts,local leukemic infiltrations.Bone marrow cells from long term remission AML patients and normal controls also showed bclXL downregulation after in vitro Vp16 treatment,but the apoptotic rates were lower than that in nonremissive AML.Conclusion:Overexpression of bclXL played an important role in leukemogenesis and drug resistance.Downregulation of bclXL was necessary but not sufficient to initiate apoptosis.

关 键 词：细胞凋亡 基因表达 白血病 髓细胞性 药物疗法 

分 类 号：R733.71[医药卫生—肿瘤]