二羟基乙二肟的细胞恶性转化研究  

A Study on Cell Malignant Transformation Caused by Dihydroxyglyoxime

作　　者：沈建英[1,2] 蒋芸[1,2] 张招弟 秦椿华[1,2] 彭德慧 

机构地区：[1]山东滨州医学院卫生学教研室 [2]同济医科大学工业毒理室

出　　处：《中华预防医学杂志》1997年第6期246-248,共3页Chinese Journal of Preventive Medicine

摘　　要：为检测二羟基乙二肟（ＤＨＧ）对细胞的恶性转化能力，判断其体外致癌性，本研究用ＤＨＧ对ＢＡＬＢ／ｃ－３Ｔ３细胞进行转化试验。结果表明：ＤＨＧ能诱导ＢＡＬＢ／ｃ－３Ｔ３细胞发生转化，转化细胞可使刀豆球蛋白（ＣｏｎＡ）凝集并可在软琼脂上生长，将其接种于ＢＡＬＢ／ｃ裸小鼠皮下能诱发出纤维肉瘤。以上结果充分说明ＤＨＧ诱导的ＢＡＬＢ／ｃ－３Ｔ３细胞的转化为恶性转化。BALB/c3T3 cell transformation test was used to detect cell carcinogenicity caused by dihydroxyglyoxime (DHG) and determine its carcinogenicity in vitro. Results showed that DHG could induce transformation of BALB/c3T3 cells and the transformed cells could agglutinate in the presence of Con A and grew in the soft agar. Transformed cells could induce the formation of fibrosarcoma if they were inoculated subcutaneously into the naked mice. It indicated sufficiently that the transformation in BALB/c3T3 cell culture was malignant, and also predicted its potential carcinogenicity in human beings.

关 键 词：二羟基乙二肟 致癌性试验 细胞恶性转化 

分 类 号：R996[医药卫生—毒理学] R730.2[医药卫生—药学]