水通道蛋白-4表达变化与急性高眼压视网膜损伤的相关性研究  

A STUDY ON THE CORRELATION BETWEEN THE CHANGES OF AQUAPORIN4 EXPRESSION AND INJURIES IN RAT RETINA UNDER DIFFERENT ACUTE OCULAR HYPERTENSION

作　　者：陈海[1] 孙善全[1] 汪克建[1] 杨美[1] 谭戈[1] 刘超[1] 

机构地区：[1]重庆医科大学神经生物学研究室,重庆400016

出　　处：《解剖学报》2008年第5期677-682,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30470608)

摘　　要：目的观察不同高眼压作用下,水通道蛋白-4(AQP4)及其mRNA在大鼠视网膜内的表达变化,以探讨AQP4与眼压异常性疾病的关系。方法采用前房加压灌注法,分别制作75cm、125cm和150cm水柱不同压力急性高眼压大鼠模型;通过HE和尼氏染色了解视网膜病理改变和神经节细胞数目变化;应用免疫组织化学、免疫荧光双标、原位杂交以及RT-PCR等技术检测不同高眼压作用下,AQP4及其mRNA在视网膜内的表达变化。结果与对照组相比,各实验组大鼠视网膜呈不同程度水肿样改变,其内层厚度增加,神经节细胞数目明显减少;AQP4及其mRNA在视网膜内的表达明显增强,其表达上调水平与眼压升高程度呈正相关,并与视网膜内层厚度增加以及节细胞数目减少相关(P<0.01)。结论急性高眼压作用下,大鼠视网膜内AQP4及其mRNA的表达明显增强,呈压力依赖性,其异常高表达可能与急性高眼压的病理损伤过程以及眼内水、电解质代谢失衡有关。Objective To investigate the changes of aquaporin 4（AQP4） and its mRNA expression in rat retina under different acute ocular hypertension （ AOH ）, so as to study the role of AQP4 in some diseases related with the aheration of ocular pressure. Methods The AOH rat models were established by perfusing isotonie salt solution into the anterior chamber of rat eyes through a connected reservoir, which was situated at 75cm, 125cm and 150cm heights to produce appropriate intraocular pressure. HE staining was performed to check the thickness and morphological changes of the retinal inner layers, and Nissl staining was used to count the number of ganglion cells in the ganglion cells layer （ GCL）. The changse of AQP4 and its mRNA expression in rat retina under different AOH were detected by immunofluorescence staining and confocal microscope, in situ hybridization and RT-PCR respectively. Results The retina was swelling and the thickness of retina inner layer was improved under different AOH, and the number of ganglion cells in GCL was decreased after the perfusion stopped. The expressions of AQP4 and its mRNA in rat retina under different AOH were significantly elevated compared with the normal control groups. This up-regulation expression had a strong correlation with the thickness changes of retinal inner layer and the decrease in the cell number of the GCL under different AOH. Conclusion The up-regulation of AQP4 and its mRNA expression in rat retina was correlated with the increased intraocular pressure. The results indicate that AQP4 may play an important role in the pathophysiological process of the retinal injury under AOH.

关 键 词：急性高眼压 视网膜 MÜLLER细胞 水通道蛋白-4 免疫荧光双标记 大鼠 

分 类 号：R774[医药卫生—眼科] R338[医药卫生—临床医学]