氟、钙、碘对大鼠甲状腺抗氧化能力的交互作用观察  

Interaction of fluorine,calcium and iodine in anti-oxidation capability of thyroid gland in rats

作　　者：邹焰[1] 申旭波[1] 王建华[1] 许洁[1] 董国宾[1] 

机构地区：[1]遵义遵义医学院预防医学教研室,贵州遵义563000

出　　处：《解放军医学杂志》2008年第9期1101-1104,共4页Medical Journal of Chinese People's Liberation Army

基　　金：贵州省教育厅基金(黔教科20022321)资助项目;遵义医学院优秀青年基金(G-005)资助项目

摘　　要：目的观察不同水平氟、钙、碘对甲状腺超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)的交互作用。方法应用2×2×2析因实验设计将80只Wistar大鼠分成8组,分别进行适中氟钙碘(NF,NCa,NI)、适中氟碘高钙(NF,NI,HCa)、适中钙碘高氟(NCa,NI,HF)、适中碘高氟钙(NI,HF,HCa)、适中氟钙低碘(NF,NCa,LI)、适中氟高钙低碘(NF,HCa,LI)、适中钙高氟低碘(NCa,HF,LI)、高氟钙低碘(HF,HCa,LI)饲料喂养。适中氟为90μg/d,高氟较适中氟高30倍;适中钙为13mg/d,高钙较适中钙高20倍;适中碘为3.5μg/d,适中碘较低碘高15倍。6个月后分别采用黄嘌呤氧化酶法、可见光分光光度法和比色法测定各组动物甲状腺SOD和CAT的活性及MDA的含量。结果氟与碘对甲状腺SOD活性存在交互作用(F=3.97,P=0.05),固定为NF和NCa时,LI组SOD活性高于NI组(t=2.36,P=0.03),固定为NF和HCa时,LI组SOD活性高于NI组(t=-3.04,P=0.01),固定为HF和LI时,HCa组SOD活性低于NCa组(t=2.36,P=0.03),固定为NI和HCa时,HF组SOD活性高于NF组(t=-2.91,P=0.01)。氟、钙与碘对CAT活性均存在交互作用(P<0.01),固定为NF和NCa时,LI组CAT活性低于NI组(t=3.27,P<0.01),固定为NF和HCa时,LI组CAT活性低于NI组(t=2.65,P=0.02),固定为HF和HCa时,LI组CAT活性高于NI组(t=-16.86,P<0.01),固定为HF和LI时,HCa组CAT活性高于NCa组(t=-14.53,P<0.01),固定为NI和HCa时,HF组CAT活性低于NF组(t=5.96,P<0.01),固定为LI和HCa时,HF组CAT活性高于NF组(t=-10.05,P<0.01)。钙与碘对MDA存在交互作用(F=8.29,P=0.01),固定因素后两组间比较均无显著性差异(P>0.05)。其他各组间无差异。结论氟、钙与碘对甲状腺SOD和CAT的作用不完全一致,且存在交互作用,但对MDA的作用影响甚微。Objective To study the interactive effects of fluorine, calcium and iodine on thyroid SOD, CAT and MDA of rats. Methods Based on 2×2×2 factorial design, 80 Wistar rats were randomly divided into 8 groups and diet with different ingredients：① moderate concentrations of fluorine （F）, calcium （Ca） and iodine （I） （NF, NCa, NI）; ②moderate concentrations of F, I and high concentration of Ca （NF, NI, HCa）; ③ moderate concentrations of Ca and I and high concentration of F （NCa, NI, HF）; ④ moderate con centration of I and high concentrations of F and Ca （NI, HF, HCa） ; ⑤ moderate concentrations of F and Ca and low concentration of I （NF, NCa, LI） ; ⑥ moderate concentration of F, high concentration of Ca and low concentration of I （NF, HCa, LI） ;⑦moderate concentration of Ca, high concentration of F and low concentration of I （NCa, HF, LI） ; and ⑧ high concentrations of F and Ca and low concentration of I （HF, HCa. LI）. The moderate concentration of F was 90μg/d, and the high concentration of F was 30 times that of rood crate concentration. The moderate concentration of Ca was 13mg/d, the high concentration of Ca was 20 times that of moderate concentration. The moderate concentration of I was 3. 5μg/d, i.e. 15 times that of low concentration. After six months, the activities of SOD and CAT, and the content of MI）A in the thyroid were determined by xanthine oxidase method, visible spectrophotometry and colorimetry. Results According to the results of factorial ANOVA, the significant interaction of F with I on SOD was found （ F=3. 97, P=0.05）. The activity of SOD in ⑤ group was significantly higher than that in ① group （t=2. 36, P=0. 03）. The activity of SOD in ⑥ group was higher than that in （2） group （t=3. 04, P=0. 01）. The activity of SOD in ⑧ group was lower than that in ⑦ group （ t= 2. 36, P=0.03）. The activity of SOD in ④group was higher than that in ② group （t=2. 91, P=0. 01 ）. A significant interaction of fluorin

关 键 词：氟 钙 碘 甲状腺 交互作用 

分 类 号：R151.4[医药卫生—营养与食品卫生学]