银杏叶片对大鼠肝纤维化的影响  

Effects of ginkgo leaves on hepatic fibrosis induced by CCl_4 in rats

作　　者：周晓倩[1] 何云[2] 程明亮[3] 

机构地区：[1]贵阳市第一人民医院消化内科,贵阳550000 [2]解放军总医院第二附属医院门诊部 [3]贵阳医学院附属医院感染科

出　　处：《解放军医学杂志》2008年第9期1117-1119,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究银杏叶片对大鼠肝纤维化的治疗效果,为其在临床上的应用提供理论依据。方法70只Wistar大鼠随机分成空白对照组(10只)和模型组(60只)。应用复合造模方法(CCl4、高脂饮食和乙醇)处理2个月后,随机杀取3只大鼠确认造模成功并留取肝组织。将造模后剩余的30只模型组大鼠再分为自然恢复组和高、低剂量治疗组3组,其中高、低剂量治疗组正常进食水且每日以银杏叶片灌胃,空白对照组及自然恢复组正常进食水,同时每日予以等量自来水灌胃。2个月后留取血清、肝组织,测血清透明质酸(HA)含量及肝组织羟脯氨酸(Hyp)含量,行组织病理学检查评价肝纤维化形成情况,行免疫组化分析TGF-β1、α-SMA表达情况。结果自然恢复组血清HA及肝组织Hyp较空白对照组显著升高(P<0.05),高、低剂量治疗组较自然恢复组显著下降(P<0.05);免疫组化结果显示自然恢复组TGF-β1、α-SMA较空白对照组升高(P<0.05),高、低剂量治疗组较自然恢复组显著下降,且TGF-β1与α-SMA之间具有正性相关(高剂量治疗组:r=0.735,P<0.05;低剂量治疗组:r=0.936,P<0.01)。结论银杏叶片可通过TGF-β1途径调节肝星状细胞(HSC)的活性而达到抗纤维化的作用。Objective To investigate the therapeutic effect of ginkgo leaves on hepatic fibrosis, so as to found a theoretical basis for the clinical application of ginkgo leaf preparation. Methods Seventy male Wistar rats were randomly assigned into normal control group （n=10） and model group （n=60）. All the rats in model group were subcutaneously given CCl4 injection, high fat and low protein diet and alcohol to establish hepatic fibrosis models. Eight weeks later, three rats were sacrificed to make sure the success of hepatic fibrosis model. The survived 30 rats in model group were again randomly divided into 3 groups： spontaneous recovery group, high dose-treated group and low dose-treated group （10 each）. Rats in the two treated groups were conventionally fed and perfused with the extracts of ginkgo leaves every day, while the rats in normal control group and spontaneous recovery group were conventionally fed and perfused with water. Eight weeks later. the liver index, levels of serum hyaluronic acid （HA） and liver hydroxyproline （Hyp） were measured, and the degree of he patic fibrosis was estimated. The expressions of TGF-β1 and α-SMA were detected respectively by imrnunohistochemistry. Results Corn pared with normal control group, the serum HA and liver Hyp increased in spontaneous recovery group （ P〈0. 05）, while decreased in the two treated groups compared with the spontaneous recovery group （P〈0. 05）. The expressions of TGF-β1 and α-SMA in spontaneous re covery group were higher than that in normal control group （P〈0.05）. Comparecl with spontaneous recovery group, the expressions of TGF-β1 and α-SMA decreased remarkably and showed a direct correlation in the two treated groups （r=0. 735 and P〈0. 05 in high dose treated group, and r=0. 936 and P〈0. 01 in low dose treated group）. Conclusion The extracts of ginkgo leaves may palliate the hepatic fibrosis by regulating the activity of hepatic stellate cells （HSC） through TGF-β1 pathway.

关 键 词：银杏 肝硬化 

分 类 号：R575.29[医药卫生—消化系统]