青春期大鼠前伸下颌后浅层嚼肌细胞膜乙酰胆碱受体研究  

Changes of the acetylcholine receptor on rat′s superficial masseter muscles after functional mandibular advancement

作　　者：黄宁[1] 陈开云[2] 罗颂椒[2] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学 [2]四川大学华西口腔医院正畸科,四川成都610041

出　　处：《华西口腔医学杂志》2008年第4期365-367,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30171024)

摘　　要：目的研究功能矫形前伸青春期大鼠下颌后,前伸下颌的浅层嚼肌细胞膜乙酰胆碱受体最大结合容量和亲和性的变化,探讨功能矫形的神经肌肉调控机制。方法选用40只5周龄SD雄性大鼠,随机分为实验组和对照组各20只。实验组大鼠戴自制上颌功能矫治器,引导下颌前伸,对照组不戴。采用放射受体分析法测定2组大鼠浅层嚼肌肌细胞膜上乙酰胆碱受体的最大结合容量和亲和性值。结果实验组大鼠浅层嚼肌细胞膜上乙酰胆碱受体最大结合容量明显增加,具有高度亲和力,大于对照组。结论功能矫形可增加浅层嚼肌细胞膜上乙酰胆碱受体与乙酰胆碱的结合量及亲和力,导致肌肉的结构与功能发生适应性改建。Objective The purpose of this study was to evaluate the maximum binding （Bmax） and affinity Kd value changes of aeetylcholine receptor（n-AehR） on rat＇s superficial masseter muscles after functional mandibular advancement. Methods Forty 5-week-old male SD rats were randomly divided into experimental and control groups. The mimic functional appliances were only used in experimental groups, and the rats of two groups were killed after 1, 3, 7, 14 days. Radio-ligand binding assay（RBA） was applied to determine the Bmax and Kd value of n-AchR on the superficial masseter muscles. Results The Bmax of n-AchR in experimental groups was higher than that in the control groups at all time points, and the differences had statistical significance. The Kd value of n-AchR was higher in experimental group than that in control groups in 1, 3, 7 days, while Kd value in experimental group of n-AchR was lower than that in control groups in 14 days. The differences had statistical significance in 7 and 14 days, but the differences had no statistical significance in 1 and 3 days. Conclusion The functional orthopedics can increase the Bmax and affinity of n-AchR on rapid growing rat＇s superficial masseter muscles.

关 键 词：功能矫形 浅层嚼肌 乙酰胆碱受体 

分 类 号：R783.5[医药卫生—口腔医学]