唾液腺黏液表皮样癌组织中分化抑制因子-1和血小板反应蛋白-1的表达  

The expression of inhibitor-1 of DNA binding/differentiation-1 and thrombospondin-1 in mucoepidermoid carcinoma of different malignant degree

作　　者：杨森[1] 李安[1] 郭丽娟[1] 于涛[1] 龚仁国[1] 许瑞升[1] 高庆红[2] 宣鸣[2] 王昌美[2] 王晓毅[2] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学 [2]四川大学华西口腔医院头颈肿瘤外科,四川成都610041

出　　处：《华西口腔医学杂志》2008年第4期425-429,共5页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(0040305401042)

摘　　要：目的探讨不同恶性程度的唾液腺黏液表皮样癌组织中分化抑制因子-1(Id-1)、血小板反应蛋白-1(TSP-1)的表达情况及相互关系。方法以不同恶性程度的唾液腺黏液表皮样癌和正常唾液腺组织为材料,采用免疫组化SP法检测正常唾液腺组织以及高分化、中分化和低分化黏液表皮样癌组织中Id-1、TSP-1基因的表达情况。结果Id-1和TSP-1在正常唾液腺中的阳性表达率明显低于在黏液表皮样癌组织中的阳性表达率(P值分别为0.000和0.013)。在中分化和低分化黏液表皮样癌组织中Id-1的蛋白表达率明显高于高分化黏液表皮样癌(P值分别为0.001和0.002),而Id-1在中分化和低分化黏液表皮样癌组织中的蛋白表达率差异无统计学意义(P>0.05)。在低分化黏液表皮样癌组织中TSP-1的蛋白表达率明显低于高分化黏液表皮样癌(P=0.014),而TSP-1在中分化和低分化黏液表皮样癌组织中的蛋白表达率差异无统计学意义(P>0.05),在中分化和高分化黏液表皮样癌组织中的蛋白表达率差异无统计学意义(P>0.05)。TSP-1的表达与Id-1呈负相关(r=-0.394,P=0.002)。结论TSP-1的表达可能抑制黏液表皮样癌的发生,而Id-1的表达则可能促进黏液表皮样癌的发生,二者之间呈负相关。Objective To study the expression of inhibitor-1 of DNA binding/differentiation-1 （Id-1） and throm- bospondin-1 （TSP-1） genes in mucoepidermoid carcinoma of different malignant degree and analyze the relationship between them. Methods Using immunohistochemistry （IHC） staining technique, TSP-1 and Id-1 proteins in the mucoepidermoid carcinoma of different malignant degree, including well-differentiated, moderately differentiated and poorly differentiated mucoepidermoid carcinoma, and normal salivary gland tissues were detected. Results The positive rate of Id-1 and TSP-1 in normal salivary glands were apparently lower than that in malignant mucoepidermold carcinoma（P=0.000, P=0.013）. The positive rate of Id-1 in moderately and poorly differentiated mucoepidermold carcinoma was higher than that of the well-differentiated （P=0.001, P=0.002）. However, the positive expression of Id-1 showed no relationship between the moderately and poorly differentiated mucoepidermoid careinoma（P〉0.05）. The positive rate of TSP-1 in poorly differentiated mucoepidermoid carcinoma was less than that of the well-differentiated（P=0.014）. The positive expression of TSP-1 showed no relationship between the moderately and poorly dif- ferentiated mucoepidermoid carcinoma（P〉0.05）, and the positive expression of it also showed no relationship between the moderately and well differentiated mucoepidermoid carcinoma （/）〉0.05）. The expression of Id-1 and TSP-1 showed negative correlation（r=-0.394, P=0.002）. Conclusion The expression of TSP-1 may inhibit the development of the mucoepidermoid carcinoma, contrarily, the expression of Id-1 may prompt the development of the mucoepider-moid carcinoma. The expression of Id-1 and TSP-1 has negative correlation.

关 键 词：黏液表皮样癌: 血小板反应蛋白-1 分化抑制因子-1 

分 类 号：R739.87[医药卫生—肿瘤]