高效液相色谱法测定茶黄酊中儿茶素、表儿茶素和黄芩苷的含量  

HPLC determination of catechin,epicatechin and baicalin in Chahuang tincture

作　　者：谭生建[1] 刘刚[1] 张锋[1] 潘敏翔[1] 张华[1] 姜韧[1] 

机构地区：[1]中国人民解放军第306医院药学部,北京100101

出　　处：《药物分析杂志》2008年第9期1474-1477,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法测定茶黄酊中儿茶素、表儿茶素和黄芩苷含量。方法:采用 Agilent ZORBAX Eclipse XDB-C_(18)(150 mm×4.6 mm,5 μm)色谱柱;流动相 A 为0.5%三乙胺溶液(以磷酸调 pH 至3.0),流动相 B 为乙腈,梯度洗脱(0～12 min,流动相 B 10%;12.1～30 min,流动相 B 22%);流速:1 mL·min^(-1),检测波长278 nm。结果:儿茶素、表儿茶素和黄芩苷线性范围分别为26.75～214.0μg·mL^(-1)(r=0.9998),5.568～44.54μg·mL^(-1)(r=0.9998),3.520～28.16μg·mL^(-1)(r=0.9999)。回收率(n=6)分别为99.8%,98.9%,102.6%;RSD 分别为0.61%,1.2%,0.92%。结论:本方法简便、准确、稳定,重复性好,可用于茶黄酊中儿茶素、表儿茶素和黄芩苷的含量测定。Objective：To develop an HPLC quantitative method for the determination of catechin, epicatechin and o baicalin in Chahuang tincture. Methods ：The chromatographic conditions include column C18 （150 mm × 4. 6 mm, 5 μm）,0.5% triethylamine（adjusted to pH 3.0 by phosphoric acid） as mobile phase A,acetonitrile as mobile phase B,gradient elution （0 - 12 min,10% for mobile phase B;12.1 -30 min,22% for mobile phase B）. The flow rate was 1 mL · min^-1 and monitored at 278 nm. Results： The linear ranges for catechin, epicatechin and baicalin were 26.75 -214.0 μg · mL^-1（r =0. 9998） ,5. 568 -44.54 μg · mL^-1（r=0. 9998） ,3. 520 -28.16 μg · mL^-1 （r = 0. 9999 ）. The average recoveries of catechin ,epicatechin and baicalin were 99.8% ,98.9% and 102. 6% with RSD of 0.61%, 1.2% ,0.92%, respectively. Conclusion：The method is simple, accurate, stable and precise.

关 键 词：高效液相色谱法 茶黄酊 儿茶素 表儿茶素 黄芩苷 

分 类 号：R917[医药卫生—药物分析学]