木聚糖酶对肉仔鸡后肠道微生物的影响  

The effects of xylanase supplementation on the hindgut microbe in broiler chickens

作　　者：高俊勤[1] 江芸[2,3] 张耀[1] 高峰[1,3] 周光宏[3] 朱伟云[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]南京师范大学金陵女子学院,江苏南京210097 [3]教育部肉品加工与质量控制重点开放实验室,江苏南京210095

出　　处：《草业学报》2008年第5期104-110,共7页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(30300251)资助

摘　　要：主要研究了小麦基础日粮添加木聚糖酶对肉仔鸡后肠道微生物的影响。将120只7日龄肉仔鸡分成2组,日粮分别添加0,0.1%木聚糖酶,饲喂至21日龄,研究木聚糖酶对肉仔鸡后肠道菌群数量变化的影响。结果表明,加酶未使回肠和盲肠乳酸杆菌、双歧杆菌和大肠杆菌数量发生显著变化。利用变性梯度凝胶电泳技术研究了木聚糖酶对肉仔鸡后肠细菌群体的影响。结果表明,加酶组回肠和盲肠的图谱比对照组条带相对较多,但差异不显著;组内个体间的图谱相似性比组间的相似性相对较大。木聚糖酶影响肉仔鸡后肠微生物数量和种群的作用效果不明显。This study examined the effects of xylanase supplementation on microbes in the hindgut of broiler chickens fed on wheat-based diets. One hundred and twenty 7-day-old broiler chickens were divided into two groups, and fed on wheat-based diets supplemented with 0 or 0.1 % xylanase. The diets were fed to chickens between 7 and 21 d of age. First, the effects of xylanase supplementation on bacterial populations in the hindgut of broiler chickens were studied by traditional methods and found that there was no significant difference between the two experimental groups in counts of lactobaeillus, bifidobacterium and coliform bacteria in the ileum and caeca （P〉0.05）. Second denaturing gradient gel electrophoresis （DGGE） showed that there were more bands from the xylanase addition group in the DGGE profiles from the ileum and caeca than in those from the control, although the differences not significant. The intra-group＇s coefficient of similarity of the DGGE promentation does not signifiea change the hindgut microflora in broiler chickens fed on wheat-based diets.

关 键 词：木聚糖酶 肉仔鸡 微生物 变性梯度凝胶电泳 

分 类 号：S831.5[农业科学—畜牧学]