RANKL、MMP-9和MMP-2在成釉细胞瘤中的表达及意义  

The expression and significance of RANKL,MMP-9 and MMP-2 in ameloblastoma

作　　者：钱永[1] 黄洪章[2] 潘朝斌[2] 张彬[2] 王建广[2] 

机构地区：[1]南昌大学第一附属医院口腔颌面外科,江西南昌330006 [2]中山大学附属第二医院口腔颌面外科,广东广州510120

出　　处：《中国口腔颌面外科杂志》2008年第5期362-367,共6页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(30471896);广东省自然科学基金(04300340)~~

摘　　要：目的:探讨RANKL、MMP-9和MMP-2与成釉细胞瘤骨吸收机制之间的关系。方法:应用免疫组化、荧光定量RT-PCR及Western印迹方法,分别在不同分子水平检测RANKL、MMP-2和MMP-9在成釉细胞瘤中的表达,所有实验数据均经SPSS 11.0统计软件包进行分析处理,组间比较采用t检验。结果:在所有成釉细胞瘤标本中,3种方法均可检测到RANKL、MMP-2和MMP-9的表达。其中,RANKL各型成釉细胞瘤中均呈恒定的高表达,而荧光定量RT-PCR检测结果显示,MMP-2在基底细胞型成釉细胞瘤中的表达水平显著高于其他两型(P<0.05)。结论:MMP- 2、MMP-9及RANKL可能共同参与成釉细胞瘤引起的骨质吸收过程。与MMP-9相比,MMP-2可能与成釉细胞瘤的侵袭性更为相关。PURPOSE： The purpose of this study was to investigate the relation between the expression of RANKL, MMP-2 and MMP-9 in ameloblastoma and bone resorption caused by ameloblastoma. METHODS： Immunohistochemistry, real time RT-PCR and Western blot were used to detect the expression of RANKL, MMP-2 and MMP-9 in diverse types of ameloblastoma. The data were analyzed using SPSS 11.0 Software package for Student＇ s t test. RESULTS： In all cases, the expression of MMP-2, MMP-9 and RANKL were detected by the three methods mentioned above. The expression of RANKL was constantly high in diverse subtypes of ameloblastoma, and the expression of MMP-2 mRNA in basal cell ameloblastoma was significantly higher than that in the other two subtypes （P〈0.05）. CONCLUSIONS： MMP-2, MMP-9 and RANKL may collectively participate in the bone resorption process caused by ameloblastoma. Compared with MMP-9, MMP-2 may be more closely correlated with invasiveness of ameloblastoma.Supported by National Natural Science Foundation of China （Grant No.30471896） and Guangdong Provincial Natural Science Foundation （Grant No. 04300340）.

关 键 词：RANKL MMP-2 MMP-9 成釉细胞瘤 骨吸收 

分 类 号：R739.8[医药卫生—肿瘤]