Chk2反义寡核苷酸对放射线照射诱导的HeLa细胞凋亡的影响  

Influence of antisense oligodeoxynucleotide targeting chk2 on apoptosis of HeLa cells induced by X ray

作　　者：朱克修[1] 李玢[1] 王佳[1] 曹亚莉[1] 韩晓兵[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科,陕西西安710061

出　　处：《现代肿瘤医学》2008年第10期1665-1668,共4页Journal of Modern Oncology

基　　金：陕西省科技计划项目[编号:2006K09-G11-(3)]

摘　　要：目的:观察宫颈癌细胞HeLa转染chk2反义寡核苷酸后,在X射线作用下对凋亡的影响。方法:以脂质体为载体将chk2正义链和反义链转染HeLa细胞,用Western blot检测HeLa细胞中Chk2蛋白的表达,用流式细胞仪和TUNEL法检测X射线照射后细胞凋亡率。结果:反义封闭chk2可明显抑制Chk2蛋白表达(P<0.01),X射线作用下细胞凋亡率明显高于转染正义链组(P<0.01)。结论:反义封闭chk2基因可增加He-La细胞对X射线照射的凋亡敏感性。Objective：To investigate the change of antisense oligodeoxynueleotide targeting chk2 apoptosis in cervical cancer cells HeLa induced by X ray. Methods： Oligonucleotide was transfected into HeLa cells by liposome.The expression of Chk2 was observed by Western blot. The apoptosis of HeLa cells induced by X ray was investigated by flow cytometer and TUNEL. Results： Transfection with antisense oligonuleotide targeting chk2 could inhibit the expression of Chk2 protein （P 〈 0.01 ）, and the apoptosis rate was significantly higher than that of the sense blocking （ P 〈 0.01 ）. Conclusion： Antisense blocking of chk2 could increase the apoptotic sensitivity of HeLa cells to X ray.

关 键 词：chk2基因 反义核酸技术 宫颈癌 放射敏感性 

分 类 号：R737.33[医药卫生—肿瘤]