胶质瘤中PI3K、AKT2和NDRG2 mRNA的表达及相关性  

Expression of PI3K,AKT2,NDRG2 mRNA and the correlation in Human Glioma

作　　者：周彬[1,2] 邓艳春[1] 刘南[1,3] 唐琢[1] 石洁[1] 曹卫东[4] 王西玲[4] 

机构地区：[1]第四军医大学西京脑科医院神经内科,陕西西安710032 [2]兰州军区临潼疗养院疗养八科,陕西西安710060 [3]解放军第451医院神经内科,陕西西安710054 [4]第四军医大学西京脑科医院神经外科,陕西西安710032

出　　处：《现代肿瘤医学》2008年第10期1675-1677,共3页Journal of Modern Oncology

基　　金：国家自然科学基金(30672168)

摘　　要：目的:研究胶质瘤中蛋白激酶PI3K、AKT2 mRNA表达与NDRG2 mRNA表达的相关性,寻找与NDRG2相互作用的上下游分子。方法:用实时定量PCR技术检测29例脑胶质瘤及相应瘤旁组织中PI3K、AKT2和NDRG2 mRNA表达量。结果:29例脑胶质瘤NDRG2 mRNA表达明显低于瘤旁组织(P<0.05),且不同病理分级间表达有显著性差异(P<0.05),其中Ⅳ级NDRG2 mRNA表达量明显低于Ⅱ级和Ⅲ级胶质瘤(P=0.007,P=0.03);AKT2,PI3K mRNA表达在胶质瘤及瘤旁组织之间无明显差异(P>0.05)。AKT2,PI3K mRNA表达与NDRG2 mRNA表达的相关性无统计学意义(P>0.05)。结论:NDRG2可能与胶质瘤的发生发展及恶性演变有关,在mRNA表达水平,还不能认为PI3K、AKT2与NDRG2之间有相关性。Objective：To investigate the expression and correlation of PI3K- AKT2 signal transduction pathway with NDRG2 in human glioma,so as to find the upstream or downstream molecular interacting with NDRG2. Methods： Real time PCR was used to analyze the expression of PI3K,AKT2 and NDRG2 mRNA in 29 glioma tissues and adjacent normal tissues. Results： Compared with adjacent normal tissues,the expression level of NDRG2 mRNA in glioma tissues was reduced significantly （0.95 ± 0. 16 vs 1.42 ± 0. 23, P 〈 0. 05）. And there was a trend for NDRG2 level to reduce with increasing pathologic grade （P 〈 0.05）. However, the expressions of AKT2 and PI3K had no obviously difference between glioma and adjacent normal tissues （ P 〉 0.05 ）. The analysis of correlation had no statistial significance among PI3K, AKT2 and NDRG2 （ P 〉 0. 05 ）. Conelution： The aberrant expression of NDRG2 is possibly associated with the malignant transformation of glioma, however,PI3K and AKT2 possibly have no correlation with NDRG2 in mRNA level in glioma.

关 键 词：神经胶质瘤 P13K AKT2 NDRG2 

分 类 号：R730.264[医药卫生—肿瘤]