地塞米松对兔视神经钳夹伤后视网膜神经节细胞存活及Nogo-A表达的影响  

Dexamethasone on retinal ganglion cells survival and Nogo-A expression after rabbit optic nerve trauma

作　　者：李晓双[1] 鞠学红[1] 王亚南 

机构地区：[1]潍坊医学院人体解剖学教研室,山东省潍坊市261042 [2]潍坊医学生理学教研室,山东省潍坊市261042

出　　处：《眼科新进展》2008年第10期737-739,共3页Recent Advances in Ophthalmology

基　　金：山东省教育厅资助项目(编号:J06L23)~~

摘　　要：目的观察地塞米松对家兔视神经钳夹伤后视网膜神经节细胞(retinal ganglion cell,RGC)存活的影响及损伤视神经、视网膜Nogo-A表达的变化。方法采用兔球后视神经钳夹伤模型,健康成年家兔分为正常对照组、损伤组、治疗组。分别于损伤后3d、7d、14d处死动物,观察单位面积RGC存活数量及损伤后Nogo-A在视神经、视网膜的表达变化。结果视神经损伤后,治疗组RGC的存活数高于损伤组及对照组(P<0.01)。对照组、损伤组损伤后3d、7d、14d视神经、视网膜Nogo-A表达增强,至损伤后7d达高峰;治疗组视神经、视网膜表达亦增强,各时间点均弱于损伤组、对照组,差异有显著统计学意义(P<0·01)。结论视神经损伤后,地塞米松能够增加RGC的存活数量,能够下调No-go-A的表达,这可能是地塞米松治疗作用机制之一。Objective To observe the effect of dexamethasone on retinal ganglial cells（RGC） survival after optic nerve trauma and the changes in expression of Nogo-A in injured optic nerve and retina. Methods Intraorbital optic nerve trauma model of rabbit was used. Healthy adult rabbits were divided into control group,injured group,and treatment group. At 3 days,7 days and 14 days after injury, the rabbits were killed respectively. RGC survival and the expression of Nogo-A in optic nerve and retina were observed after injured. Resuits After optic nerve trauma, the numbers of survived RGC in treatment group were higher than those in control group and injured group（ P 〈0. 01 ）.At 3 days,7 days and 14 days after injured,the expression of Nogo-A increased in optic nerve and retina in control group and injured group ,and reached to the peak at 7 days after injured. The expression of Nogo-A also increased in optic nerve and retina in treatment group, but was less than that in control group and injured group（P 〈 0.01 ）. Conclusion After optic nerve injured, dexamethasone can increase the number of survived RGC and can down regulate the expression of Nogo-A, which maybe one of its possible therapeutic effects.

关 键 词：地塞米松 视神经 视网膜 损伤 NOGO-A 

分 类 号：R774.6[医药卫生—眼科]