川芎嗪联合氟尿嘧啶对胃癌细胞SGC-7901/ADR的杀伤作用  

Lethal Effect of Combination of Tetramethylpyrazine and Fluorouracil in Multidrug Resistance of Gastric Carcinoma Cell Lines SGC-7901/ADR

作　　者：杨叶[1] 侯培珍[1] 张娟[1] 

机构地区：[1]内蒙古包头医学院第一附属医院急诊科,014010

出　　处：《肿瘤防治研究》2008年第9期624-626,共3页Cancer Research on Prevention and Treatment

摘　　要：目的观察川芎嗪(TMP)联合氟尿嘧啶(5-Fu)对胃癌多药耐药细胞SGC7901/ADR细胞的体外杀伤作用。方法采用MTT法观察TMP联合5-Fu对SGC7901/ADR细胞的杀伤作用,光镜下观察并采用流式细胞仪(FCM)测定各药物组细胞周期的分布。结果5-Fu对SGC-7901/ADR细胞的半数抑制率(IC50)为13.001mg/L,与300mg/LTMP联合后,使其的IC50降至1.542mg/L,逆转倍数是8.43倍,与对照组相比差异具有统计学意义(P<0.01)。光镜下可见5-Fu+TMP(终浓度300mg/L)组较5-Fu组癌细胞皱缩变小变圆,出现凋亡改变。FCM分析:TMP联合5-Fu与对照组相比将细胞阻滞在DNA合成前期和静息期-G0/G1期(P<0.05)。结论TMP与5-Fu联合对胃癌多药耐药细胞SGC7901/ADR有较强的杀伤作用,为当前胃癌治疗提供了新思路。Objective To investigate the lethal effect of combination of TMP and 5-Fu in the multidrug resistance of Gastric Carcinoma Cell Lines SGC-7901/ADR. Methods Gastric carcinoma cell lines SGC- 7901 and the multidrug resistance cell lines SGC-7901/ADR were considered as target cells, MTT assay was used to investigate the lethal effect of combination of TMP and 5-Fu in vitro, which was observed by Light microscope. Flow cytometer（FCM） was employed to investigate the cell cycles. Results The half inhibition ratio（IC50 ）of 5-Fu against SGC-7901/ADR was 13. 001 mg/L, which was reduced to 1. 542 mg/L by 300 mg/L TMP, The reversal index was 8. 43 （P〈0. 01 ）. Malignant cell volume and morphous which exposed to 5-Fu ＋ TMP became smaller and strinked rounder by Light microscope. FCM revealed that with the effect of 5-Fu ＋ TMP, cell cycle was blocked at presynthetic phase and ambiguous phase-G0/ G1 phase and The cells enter the period of DNA synthesis were reduced, inhibition mitosis of SGC-7901/ ADR（P〈0. 05）. Conclusion Combination of 5-Fu and TMP has stronger lethal effect against the multidrugs resistance Cell Lines SGC-7901/ADR in Gastric Carcinoma, which may provid a new therapeusis against Gastric Carcinoma.

关 键 词：胃癌 多药耐药性 SGC7901/ADR细胞 5-Fu 川芎嗪 

分 类 号：R735.2[医药卫生—肿瘤] R73-36[医药卫生—临床医学]