BRD7、NGX6基因在急性白血病中的表达  

Expression of BRD7 and NGX6 Gene in Acute Leukemia

作　　者：唐雪元[1] 龙潺[1] 刘芬[2] 吕宏伟[3] 胡进[3] 沈守荣[2] 

机构地区：[1]中南大学湘雅三医院血液科,长沙410013 [2]中南大学湘雅三医院消化科,长沙410013 [3]中南大学湘雅三医院医学实验中心,长沙410013

出　　处：《肿瘤防治研究》2008年第9期635-638,共4页Cancer Research on Prevention and Treatment

基　　金：湘雅三医院博士化基金资助项目(2005);湖南省卫生厅课题资助项目(C2006-20)

摘　　要：目的探讨急性白血病患者骨髓单个核细胞BRD7、NGX6基因的表达水平。方法采用RT-PCR对52例急性白血病(acute leukemia,AL)患者及30例正常骨髓对照进行NGX6、BRD7基因mR-NA水平的检测,对其中7例AL患者用免疫组化法检测BRD7蛋白表达水平。结果在AL患者和正常骨髓对照的骨髓单个核细胞中BRD7、NGX6基因均存在明确表达。NGX6 mRNA的相对表达量在AL组与正常对照中差异无统计学意义(t=-1.014,P=0.982)。而BRD7 mRNA的表达水平AL组高于正常对照组,差异有统计学意义(t=3.672,P=0.001),但在AL各亚型之间BRD7 mRNA的表达差异无统计学意义(t=1.076,P=0.287),且BRD7 mRNA的表达水平与患者初诊时骨髓原始细胞数、外周血白细胞数及血小板数无关。其中,对部分样品检测了BRD7蛋白的表达,结果发现AL患者BRD7阳性表达细胞百分率均值高于正常骨髓对照。结论AL患者和正常骨髓对照的骨髓单个核细胞均表达BRD7和NGX6,且BRD7基因在急性白血病细胞中表达上调,但BRD7 mRNA表达水平与AL患者某些临床特征无相关性。Objective To investigate the expression levels of BRD7 and NGX6 gene in bone marrow cells from adult patients with acute leukemia（AL）. Methods The bone marrow mononuclear cells （BMNC） obtained from 52 patients with acute leukemia（AL） and 30 normal bone marrow subjects as controls were separated by density gradient eentrifugation. The mRNA levels of BRD7/NGX6 in the BMNCs were detected by RT-PCR. The expression of BRD7 protein in 7 patients with AL was assayed by immunohistiochemical stains. Results The mRNA expression of BRD7/NGX6 in BMNCs from patients with AL and controls were detected. The mRNA relative expression of NGX6 gene was no significant difference in both AL and the control group（t = - 1. 014,P = 0. 982）. The mRNA expression of BRD7 in patients with AL was higher than that in the control group, and the difference was significant（t = 3. 672, P = 0. 001 ）, but there was no significant difference in ALL, M1, M2, M3, M4 and M5 subtypes of ANLL（t = 1. 076, P = 0. 287）. The mRNA expressions of BRD7 gene in patients with AL at onset were not correlated with blast cells rates in bone marrow cells, and the same as platelets and white blood cells counts in total peripheral blood. The protein expression of BRD7 was detected in part samples, and there were high per eentage of BRD7 protein positive cells in patients with AL. Conclusion The expression of BRD7/NGX6 were detected in BMNCs from patients with AL and normal subjects, and the expression of BRD7 was up-regulated in AL cells. But the mRNA expression level of BRD7 gene was not correlated with some clinical character of patients with AL at onset.

关 键 词：白血病 BRD7 NGX6 基因表达 

分 类 号：R733.71[医药卫生—肿瘤]