LivinmRNA及Bcl-2,p53蛋白在胰腺癌中的表达  

Expression of LivinmRNA and Bcl-2,p53 proteins in pancreatic carcinoma

作　　者：刘源[1] 白洁[1] 耿东华[1] 刘金钢[1] 

机构地区：[1]中国医科大学附属盛京医院普通外科,辽宁沈阳110004

出　　处：《中国普通外科杂志》2008年第9期870-873,共4页China Journal of General Surgery

摘　　要：目的探讨凋亡抑制蛋白Livin在胰腺癌组织中的表达及其与Bcl-2和p53蛋白表达的关系。方法采用逆转录-多聚酶链反应(RT-PCR)法检测52例胰腺癌和12例正常胰腺组织中Livin mRNA的表达;用免疫组织化学法检测胰腺癌组织中Bcl-2和p53蛋白的表达。结果Livin mRNA在胰腺癌中的表达率为71.2%,明显高于正常胰腺组织(P<0.01)。Livin基因表达与胰腺癌的分化程度和淋巴结转移有关(P<0.05)。Livin基因表达与Bcl-2蛋白表达有关,而与p53蛋白无关。结论Livin基因在胰腺癌的发生发展中起重要作用;Livin与Bcl-2的异常表达可能在胰腺癌的发生中起协同作用;Livin基因过表达与抑癌基因p53的失活无关。Objective To study the expression of the apoptosis inhibitor Livin gene and its relationship with the expression of Bcl-2, p53 proteins in pancreatic carcinoma. Methods The expression of Livin mRNA was investigated by reverse transcriptase polymerase chain reaction （RT-PCR） in pancreatic carcinoma tissue of 52 cases and normal pancreatic tissue of 12 cases. The expression of Bcl-2 and p53 was detected by immunohistochemical assay. Results The expression rate of Livin gene in pancreatic carcinoma tissue was 71.2% , which was obviously higher than that in normal pancreatic tissue （ P 〈 0. 01 ）. There was relationship between Livin gene expression and the differentiation and lymph node metastasis of pancreatic carcinoma （ P 〈 0.05 ）. The expression of Livin gene was correlated with Bcl-2 expression, but was not with p53 expression. Conclusions Livin gene may play an important role in the pathogenesis of pancreatic carcinoma. The up-regulated expression of Livin and Bcl-2 may play synergetic roles in the process of carcinogenesis of pancreatic carcinoma. There was no relationship between Livin expression and dysfunction of p53.

关 键 词：胰腺肿瘤/遗传学 胰腺肿瘤/病理学 基因表达 

分 类 号：R735.9[医药卫生—肿瘤]