Characterization of microRNAs in serum： a novel class of biomarkers for diagnosis of cancer and other diseases  

作　　者：Xi Chen Yi Ba Lijia Ma Xing Cai Yuan Yin Kehui Wang Jig ang Guo Yujing Zhang Jiangning Chen Xing Guo Qibin Li Xiaoying Li Wenjing Wang Yan Zhang Jin Wang Xueyuan Jiang Yang Xiang Chen Xu Pingping Zheng Juanbin Zhang Ruiqiang Li Hongjie Zhang Xiaobin Shang Ting Gong Guang Ning Jun Wang Ke Zen Junfeng Zhang Chen-Yu Zhang 

机构地区：[1]jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, China [2]Tianjin Medical University Cancer Institute and Hospital, Tianjin 300000, China [3]Beijing Genomics Institute, Shenzhen 518083, China [4]Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China [5]Department of Endocrine and Metabolic Diseases, Ruijin Hospital Affiliated to The Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China [6]Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China

出　　处：《Cell Research》2008年第10期997-1006,共10页细胞研究（英文版）

基　　金：Acknowledgments We thank Drs Fengyong Liu and Sheng Luan at UC Berkeley, USA, for their discussion and help with the writing of the manuscript. This work was supported by grants from the National Natural Science Foundation of China （no. 30225037, 30471991, 30570731）, National Basic Research Program of China （973 Program） （no. 2006CB503909, 2004CB518603）, the ＂111＂ Project, and the Natural Science Foundation of Jiangsu Province （no. BK2004082, BK2006714）.

摘　　要：Dysregulated expression of microRNAs （miRNAs） in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.

关 键 词：serum-microRNA expression profile FINGERPRINT blood-based biomarker Solexa CANCERS diabetes 

分 类 号：R44[医药卫生—诊断学]