PCR-RFLP与特异引物PCR对副溶血性弧菌进行分型鉴定探讨  

PCR-restriction fragment length polymophism and PCR methods using specific primers for typing and identification of Vibrio parahaemolyticus

作　　者：胡玉山[1] 郑桂丽[1] 胡肖娟[1] 刘俊华[1] 庞杏林[1] 邓志爱[1] 陈守义[1] 

机构地区：[1]广州市疾病预防控制中心,广州510080

出　　处：《中国卫生检验杂志》2008年第10期1977-1978,2011,共3页Chinese Journal of Health Laboratory Technology

基　　金：广州市医药卫生科技项目(2006YB137);广东省医药卫生科技项目(A2006562);广州市科技计划项目(2007J1-C0201);广州市卫生局重点专项基金(2006ZDI11)

摘　　要：目的:探讨在流行病学溯源方面,运用PCR-RFLP与特异引物PCR对副溶血性弧菌进行分型鉴定的可行性。方法:选择副溶血性弧菌核糖体基因16SrRNA序列进行PCR-RFLP分析、保守核糖体基因间隔序列16-23SrRNA(RS)和肠道细菌重复内显子保守序列(ERIC)进行特异引物PCR分析。结果:16SrRNA PCR-RFLP分析有4种模式图,RS-PCR分析有3种模式图,ERIC-PCR分析有10种模式图。结论:PCR-RFLP与特异引物PCR可以对副溶血性弧菌进行快速分子分型鉴定,有利于流行病学溯源。Objective：To study the feasibility of applying PCR-Restriction fragment length polymophism and PCR methods using specific primers for typing and identification of Vibrio parahaemolyticus for epidemiological purposes.Methods：PCR-RFLP analysis for ribosomal gene 16SrRNA and PCR methods using specific primers analysis for served ribosomal gene spacer gene sequence（RS） and enterobacterial repetitive intergenic consensus sequence（ERIC） of Vibrio parahaemolyticus were conducted.Results：There were 4 patterns using PCR-RFLP analysis for 16SrRNA.There were 3 patterns using RS-PCR analysis for RS.There were 10 patterns using ERIC-PCR analysis for ERIC.Conclusion：PCR-RFLP and PCR methods using specific primers can be used to type and identify Vibrio parahaemolyticus.It′s good for tracing epidemic pathogen.

关 键 词：副溶血性弧菌 特异引物PCR 

分 类 号：R378.3[医药卫生—病原生物学]