羟喜树碱在玻碳电极上的直接电化学行为研究  

Electrochemical behavior of hydroxycamptothecin on a glassy carbon electrode

作　　者：曲永红[1] 李丽萍[2] 刘爱林[2] 

机构地区：[1]福建医科大学成人教育学院,福州350004 [2]福建医科大学药学院,福州350004

出　　处：《海峡药学》2008年第9期107-110,共4页Strait Pharmaceutical Journal

基　　金：福建省科技重点基金资助项目(2008Y0045);福建省青年人才基金资助项目(2006F3058)

摘　　要：目的建立直接电化学检测羟喜树碱含量的方法。方法本文采用伏安法研究羟喜树碱在玻碳电极上的直接电化学行为。在磷酸盐缓冲液中(pH5.0),在-0.2～+0.4V范围内羟喜树碱在玻碳电极表面是受吸附控制,发生准可逆两电子转移电极反应过程,电子转移系数α=0.479;并以差示脉冲伏安法建立了检测羟喜树碱含量的新方法。结果在富集电位+0.8V,富集时间30s,利用差示脉冲伏安法可测得其氧化峰电流Ip与浓度分别在2.0×10-7～5.0×10-6mol.L-1内呈良好的线性关系,最低检出限为5.0×10-8mol.L-1。结论本法操作简单、快速、灵敏、准确,可为羟喜树碱药物质量的控制和检测提供了一种简便的方法。OBJECTIVE To develop a direct electrochemical method for hydroxycamptothecin （HCPT）. METHODS The electrochemical behavior of HCPT was studied by voltammetry with a glassy carbon electrode. The results exhibited the well-defined redox peak of HCPT was observed and the electrode process was adsorp- tion-controlled. The charge transfer coefficient （a） was calculated as 0. 479 in phosphate buffer solution （pH 5.0） . RESULTS The relationships between oxidation peak current and the concentration of HCPT were linear in the range of 2.0 ×10^- 7 ～ 5.0×10^-6mol·L^-1 by Differential Pulse Voltammetric method. The detection limit had been estimated as 5.0 ×10^-6mol·L^-1. This method can be used for the determination of HCPT with satisfactory results.

关 键 词：羟喜树碱 玻碳电极 循环伏安法 生物碱类化合物 

分 类 号：R914[医药卫生—药物化学]