基于不同引物的湖泊沉积物中细菌群落结构多样性的比较研究  

Study on the bacterial diversity in lake sediments based on different primers

作　　者：赵兴青[1] 杨柳燕[1] 陈灿[1] 于振洋[1] 彭乃莹 肖琳[1] 尹大强[1] 

机构地区：[1]污染控制与资源化研究国家重点实验室,南京大学环境学院,南京210093

出　　处：《南京大学学报（自然科学版）》2008年第3期289-296,共8页Journal of Nanjing University（Natural Science）

基　　金：“973”项目(2002CB412307);国家重大水环境专项(2002AA601011);国家自然科学基金(40371102)

摘　　要：采用PCR-DGGE技术，用两对引物对湖泊沉积物细菌群落结构进行了比较研究．结果表明：沉积物样品中细菌群落结构显示了明显的空间分布多样性差异，随着沉积深度的增加，细菌群落多样性指数降低．采用不同引物扩增的产物DGGE图谱聚类相近，均分成0～20cm和20～45cm两大类，但引物341f／534r得出的相似性Cs值在30．2％～75．9％之间，引物341f／926r得出的相似性Cs值在34．3％～84．1％之间，采用不同引物不同沉积深度样品群落结构之间的相似性和优势种动态变化存在差异．The bacterial community of lake sediment samples were comparatively analyzed with two sets of universal primers by PCR-DGGE, GE. The results indicated that the successional shifts in community structure were more evident at greater sediment. The Shannon-Weiner index （H） decreased along with the depth of sediment. Principle coordinate analysis suggested that the bacterial communities along the sediment core with different primers could be separated into two same groups which located 0-20 cm and 21-45 cm respectively. On the basis of Dice Coefficient （Cs）, similarity analysis of the DGGE band patterns indicated that the composition of bacterial community was discrepancy at different layers of the sediment samples, Cs between the top and the bottom layers were 30.2%- 75.9% with the primers of 341f/534r and 34. 30%- 84.1% with the primers of 341f/926r respectively. The community diversity and dominant species dynamics were obviously different based on the different sets of universal primers by DGGE.

关 键 词：沉积物 细菌多样性 变性梯度凝胶电泳(DGGE) 16S RDNA 通用引物 

分 类 号：Q143[生物学—生态学]