犬干扰素-γ的稀释复性及活力测定  

Renaturation and activity determination of recombinant canine interferon-γ inclusion body by dilution

作　　者：陈忠广[1] 张桂红[1] 夏春丽[1] 李宝贤[1] 李一经[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第9期82-86,共5页Journal of Northeast Agricultural University

摘　　要：文章探讨了不同折叠促进剂、加样方式、温度、pH在稀释法复性条件下对重组犬IFN-γ体外折叠的复性影响。结果表明,0.5mol·L-1精氨酸、0.5mol·L-1盐酸胍、2.0mol·L-1尿素均能有效抑制复性过程中蛋白质的聚集,其中精氨酸的效果最好。在4℃、pH8.0、精氨酸浓度0.5mol·L-1,蛋白浓度0.15mg·mL-1及脉冲加样操作方式下,复性后重组犬IFN-γ的活性为1.35×104U·mL-1,比活为3.74×106U·mg-1。Effects of folding enhancer, ways of adding sample, temperature and pH were investigated in the renatutation process of recombinant canine interferon-gamma inclusion body by dilution. The results showed that 0.5 mol·L^-1 L-arginine, 0.5 mol·L^-1 guanidine-HC1 and 2.0 mol·L^-1 urea could suppress the aggregation of protein during the renaturation process effectively, and 0.5 mol·L^-1 L-arginine was the best of them. The activity and the specific bioactivity were up to 1.35×10^4 U ·mL^-1 and 3.74×10^6 U·mg^-1, respectively under 4 ℃, pH 8.0, 0.5 mol·L^-1 L-arginine, 0.15 mg·mL^-1 proein and pulse renturation operation mode.

关 键 词：重组犬IFN—γ 稀释复性 折叠促进剂 活性 比活 包涵体 

分 类 号：Q511[生物学—生物化学]