p21^(waf1)基因转染对乳腺癌细胞增殖活性的影响  

Effect of p21^(waf1) gene transfection on the proliferation of human breast carcinoma cells

作　　者：宋洪生[1] 贾伟丽[2] 种瑞峰[1] 李福年[1] 刘相萍[3] 王新刚[1] 王宇[1] 

机构地区：[1]青岛大学医学院附属医院乳腺病诊疗中心,青岛266003 [2]青岛大学医学院附属青岛市立医院,青岛266011 [3]青岛大学医学院附属医院分子生物实验室,青岛266003

出　　处：《中华乳腺病杂志（电子版）》2008年第5期27-30,共4页Chinese Journal of Breast Disease(Electronic Edition)

摘　　要：目的探讨p21waf1基因转染对人乳腺癌细胞增殖活性影响及意义。方法应用脂质体介导法将pIRES-p21waf1真核表达载体转染入人乳腺癌MCF-7细胞系,并得到稳定的表达。通过荧光定量PCR、Western blot法分别检测p21waf1基因表达及蛋白表达情况,MTT法检测细胞增殖,流式细胞仪检测细胞周期分布。结果p21waf1基因转染后乳腺癌细胞生长显著受抑制,细胞周期停滞于G1期,转染组G1期、S期和G2/M期细胞比例分别为(70.52±3.21)%、(16.38±1.17)%、(9.42±0.98)%,未转染组则为(49.84±1.58)%、(36.83±1.36)%、(15.64±1.09)%,二者相比差异有统计学意义。结论p21waf1基因转染能够抑制人乳腺癌细胞周期演进和增殖活性,进而阻止乳腺癌的发生、发展,为乳腺癌治疗的一种新手段。Objective To explore the role ofp21wafl gene transfection in the proliferation of human breast carcinoma cells. Methods p21wafl gene packaged with liposome was transfected into the cells of a human breast carcinoma cell line MCF-7 ,which stably expressing p21wafl gene. The cell growth was detected by MTr. The cell cycle pattern was assayed by flowcytometry. Results After transfeetion of plRES-p21wafl , p21wafl mRNA and the protein of MCF-7-p21wafl were highly expressed compared with control. The cell growth was obviously inhibited and resulted in an accumulation of cells in Gl , presenting that the proportion of the cells in G phase was obviously increased from （49.84 ± 1.58 ） % to （ 70.52 ± 3.21 ） % , and that of the cells in S and G：/M phases was obviously decreased from （36.83 ± 1.36） % to （ 16.38 ±1.17 ） % and from （ 15.64 ± 1.09） % to （9.42 ±0.98 ） %, respectively, as compared with the control group. Conclusion p21wafl gene transfection can inhibit the growth of human breast carcinoma cells and block the cell cycle, which then inhibit the genesis and development of breast cancer. So it may become a new method to treat breast cancer.

关 键 词：基因转染 p21wafl 细胞增殖 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]