外源性SOD对兔急性高眼压致视网膜损伤保护作用的研究  

Protective effects of exogenous superoxide dismutase on rabbit retinal injury by acute intraocular hypertension

作　　者：白禹诗[1] 皮静波 孙贵范 

机构地区：[1]中国医科大学第一临床学院眼科

出　　处：《中华眼科杂志》1997年第6期429-432,共4页Chinese Journal of Ophthalmology

摘　　要：目的探讨高眼压后活性氧自由基对视网膜组织的损伤情况及外源性超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）对高眼压致视网膜损伤的保护作用。方法观察６．６７ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ）、维持１．５ｈ的高眼压解除２４ｈ内兔视网膜组织中脂质过氧化产物丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）含量、ＳＯＤ活性、谷胱甘肽过氧化物酶（ｇｌｕｔａｔｈｉｏｎｅｐｅｒｏｘｉｄａｓｅ，ＧＳＨ－Ｐｘ）活性和还原型谷胱甘肽（ＧＳＨ）含量的变化情况及球后注射Ｃｕ－Ｚｎ－ＳＯＤ对高眼压解除后１２ｈ时兔视网膜组织中ＭＤＡ含量和ＳＯＤ活性的影响。结果ＭＤＡ在高眼压解除后０～１２ｈ逐渐增加，１２～２４ｈ维持较高水平。ＳＯＤ活性和ＧＳＨ－Ｐｘ活性在高眼压解除即刻均低于正常水平，以后有不同程度增高，其中ＳＯＤ活性在高眼压解除４ｈ后又开始下降。ＧＳＨ在高眼压解除后２４ｈ内无明显变化。球后注射Ｃｕ－Ｚｎ－ＳＯＤ能减少兔视网膜组织中ＭＤＡ生成，增加ＳＯＤ活性。结论活性氧自由基参与了高眼压致视网膜损伤，球后注射大剂量ＳＯＤ对提高视网膜抗氧化损伤能力有积极意义。Objective To study active oxygen and free radical injury in rabbit retina during elevated intraocular pressure and the protective effect of exogenous superoxide dismutase (SOD) on the retinal damage by the hypertension. Methods Lipid peroxidative product, malondialdehyde (MDA), activity of SOD and glutathione peroxidase (GSHPx), reduced GSH in the retinal tissue were measured during 24 h after the release of an ocular hypertension, 6.67 kPa (1 kPa=7.5 mmHg) maintaining for 1.5 h, and the effects of retrobulbarly injected CuZnSOD on the level of MDA and the activity of SOD in the retinal tissue after the release of ocular hypertension for 12h were observed. Results MDA increased gradually during 0～12 h after the release of ocular hypertension and maintained at a relatively high level in 12～24 h. The activity of SOD and GSHPx was lower than normal level immediately after the release, and then increased to a certain different extent. But the activity of SOD began to decrease gradually 4 h after the release. GSH had no significant changes during 24 h after the release. Retrobulbar injection of CuZnSOD reduced the production of MDA in the retinal tissue and enhanced SOD activity. Conclusions Active oxygen and free radicals participate the rabbit retinal injury by elevated intraocular pressure. A high dose of CuZnSOD retrobulbar injection plays a beneficial role in enhancing the antioxidative ability of the retina.

关 键 词：高眼压 视网膜损伤 SOD 青光眼 

分 类 号：R775[医药卫生—眼科] R774[医药卫生—临床医学]