绞股蓝总皂甙对P388细胞的作用及机制探讨  

THE EFFECT OF GYPENOSIDES ON LEUKEMIA P388 CELLS AND ITS MECHANISM STUDY

作　　者：白元让[1] 魏婉丽[1] 茹祥斌[2] 李凤仙[2] 

机构地区：[1]西安医科大学临床药理研究所,西安710061 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《西安医科大学学报》1997年第3期365-367,共3页Journal of Xi'an Medical University(Chinese)

摘　　要：用单层平皿软琼脂克隆法证明绞股蓝总皂甙对体外培养的P388细胞克隆生长有明显的抑制作用，其IC50为（22．5±1．0）mg·L-1。为进一步探讨其抑制P388细胞生长机理，用放射性同位素掺入法观察了绞股蓝总皂成对P388细胞DNA、RNA及蛋白质合成的影响。结果表明，在绞股蓝总皂甙作用24h内，其主要抑制P88细胞DNA合成，轻度抑制RNA合成，而对蛋白质合成几乎无影响。Gypenosides (GP) inhibited cell growth of leukemia P388 in vitro by using cellular colony-forming assay method. The 50% inhibitory concentration of cell growth was (22. 5±1. 0)mg'L-1 respectively. In order to studv its inhibitory mechanism, radioactive isotope was incorporated in GP-supplemented 24 hours P388 cells. The results showed that GP mainly decreased DNA synthesis of P388 cells, slightly inhibited RNA synthesis and al-most had no effect on protein synthesis.

关 键 词：绞股蓝总皂甙 白血病 细胞培养 P388细胞 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]