脑出血后微管相关蛋白Doublecortin的表达及其意义  

Doublecortin expression in adult rats after intracerebral hemarrhage

作　　者：杨树旭[1] 钱聪[1] 何瞻[1] 宋正飞[1] 王义荣[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院神经外科、浙江大学邵逸夫临床医学研究所,杭州310016

出　　处：《中华神经外科杂志》2008年第9期710-712,共3页Chinese Journal of Neurosurgery

基　　金：浙江省自然科学基金资助项目（Y205055）

摘　　要：目的研究成年大鼠脑出血模型中发生的神经细胞再生现象及其特点。方法应用雄性Sprague—Dawley大鼠制作脑出血模型，采用蛋白印迹分析、免疫组化染色等方法对5-溴脱氧尿嘧啶核苷（Bromodeoxyuridine，Brdu）及未成熟神经元标记doublecortin（DCX）等进行分析。结果DCX的表达于脑出血后7d开始增加，2周后同侧脑室下区（SVZ）及基底节区可见大量DCX阳性细胞，其表达达到高峰，为对照组的（793±192）％（P〈0．01）。结论成年大鼠中SVZ的神经元前体细胞在脑出血后明显增生，并向受损伤区域移行。Objective To investigate neurogenesis in adult rat after intracerebral hemorrhage. Method Male Sprague-Dawley rats received an intracerebral infusion of 100μl autologous blood. Western blot analysis and histological examination were performed to check doublecortin （ DCX ） and Bromodeoxyuridine（Brdu）. Results DCX levels in the ipsilateral basal ganglia started to increase as early as seven days after intracerebral hemorrhage （ ICH）, peaked at 14 days （793±192 % of sham, P 〈 0.01 ）. We found that most DCX positive cells were Brdu positive. Conclusion The results demonstrated that neurogenesis occurs from subventricular zone（SVZ） in the brain following ICH.

关 键 词：脑出血 大鼠 神经细胞再生 DOUBLECORTIN 

分 类 号：R686[医药卫生—骨科学]