他扎罗汀对Colo-16细胞体外增殖的影响及其机制的研究  

In vivo study of tazarotene on the proliferation of Colo-16 cell and the relevent mechanism

作　　者：魏志平[1] 李圆[1] 刘彦群[1] 

机构地区：[1]徐州医学院附属医院皮肤科,江苏徐州221002

出　　处：《临床皮肤科杂志》2008年第10期635-638,共4页Journal of Clinical Dermatology

摘　　要：目的:研究他扎罗汀对体外培养的皮肤鳞状细胞癌细胞株Colo-16细胞体外增殖的影响,并探讨其机制。方法:用不同浓度的他扎罗汀处理Colo-16细胞,四甲基偶氮唑蓝(MTT)法测定Colo-16细胞增殖活性;免疫组化染色技术及免疫印迹法分别测定Colo-16细胞Bcl-2、Bax蛋白表达水平。结果:不同浓度的他扎罗汀对Colo-16细胞体外增殖均具有抑制作用,其抑制作用呈明显的时效和量效关系(P<0.05);他扎罗汀作用Colo-16细胞后,Bcl-2蛋白表达明显下调,Bax表达明显增强。结论:他扎罗汀能抑制皮肤鳞状细胞癌Colo-16细胞的增殖,其机制与Bcl-2蛋白表达下调和Bax表达增加有关。Objective： To investigate the influence of tazarotene on cultured cutaneous squamous cell line-Colo-16 and its possible mechanism. Methods： Colo-16 cells were treated with different concentrations of tazarotene. Cell viability was detected by MTT assay. The protein expression of Bel-2 and Bax was detected by immunocytochemistry and Western-blotting. Results： Tazarotene significantly inhibited the proliferation in a time-and dose-dependent fashion （P〈 0.05）. Bcl-2 expression was significantly reduced after tazarotene intervention, whereas Bax expression markedly enhanced. Conclusions： Tazarotene could inhibit Colo-16 cell proliferation, the reduced expression of Bcl-2 and increased expression of Bax may be involved in the process.

关 键 词：他扎罗汀 癌 鳞状细胞 Colo-16细胞 蛋白 Bcl-2 蛋白 BAX 

分 类 号：R730.261[医药卫生—肿瘤] Q786[医药卫生—临床医学]