双光子激光共焦扫描显微技术在环境化学中的应用及展望  

Applications and Prospects of Two-photon Laser Confocal Scanning Microscopy in Environmental Chemistry

作　　者：王萍[1] 朱亚先[2] 张勇[1] 

机构地区：[1]厦门大学近海海洋环境科学国家重点实验室环境科学研究中心,厦门361005 [2]厦门大学化学系,厦门361005

出　　处：《分析化学》2008年第10期1447-1452,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20777062);国家自然科学基金(创新研究群体科学基金)(No.405210003)资助项目

摘　　要：介绍了双光子激光共焦扫描显微技术的基本原理,评述了该技术与传统荧光显微技术和单光子激光共焦扫描显微技术的异同,并且结合双光子激光共焦扫描显微技术在实际工作中的应用,评述了其在环境化学中的应用潜力及发展前景。The basic principle of two-photon laser confocal scanning microscopy （TPLCSM） was introduced. Moreover, the differences between TPLCSM and traditional fluorescence microscopy, as well as the differences between TPLCSM and the single-photon laser confocal scanning microscopy （SPLCSM） were explained. In addition, the applications and prospects of TPLCSM in environmental chemistry were described based on the experimental results obtained in our laboratory.

关 键 词：双光子激光共焦扫描显微镜 环境化学 评述 

分 类 号：X13[环境科学与工程—环境科学]