糖尿病大鼠睾丸组织黄嘌呤氧化酶、髓过氧化物酶和线粒体琥珀酸脱氢酶的变化和灵芝孢子粉的干预  

Intervention Effect of Ganoderma Lucidum Spores on the Changes of XOD,MPO and SDH in the Testis Tissue of NIDDM Rats

作　　者：王淑秋[1] 秦文波[2] 康玉明[3] 马小茹[1] 刘蕾[1] 刘君星[1] 张婷[1] 张波[1] 梁衍峰[1] 王芳芳[1] 

机构地区：[1]佳木斯大学基础医学院病理生理学教研室,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院泌尿外科,黑龙江佳木斯154007 [3]山西医科大学生理教研室,山西太原030001

出　　处：《中华男科学杂志》2008年第9期792-795,共4页National Journal of Andrology

摘　　要：目的:探讨糖尿病大鼠睾丸组织黄嘌呤氧化酶(XOD)、髓过氧化物酶(MPO)和线粒体琥珀酸脱氢酶(SDH)的变化及灵芝孢子粉对睾丸组织的保护作用。方法:将清洁级Wistar大鼠50只,随机分成3组(对照组10只、模型组20只、灵芝孢子粉组20只)。模型组及灵芝孢子粉组以25mg/kg的剂量一次性腹腔注射2%链脲佐菌素,对照组腹腔注射等量柠檬酸钠-柠檬酸缓冲液。正常饮食喂养2周后,进行糖耐量试验,模型组和灵芝孢子粉组糖耐量异常者保留,余去除,并改喂高脂高糖饮食,灵芝孢子粉组另加灵芝孢子粉[250mg/(kg.d)]持续10周,动物均单独喂养,实验结束前1d做糖耐量试验,取睾丸。检测睾丸组织XOD、MPO和线粒体SDH。结果:模型组睾丸组织的SDH较对照组和灵芝孢子粉组明显降低(P<0.05),而XOD和MPO明显高于对照组和灵芝孢子粉组(P<0.05)。模型组生精小管层次不清,精子生成量减少或消失,管腔不规则,腔周围纤维组织(间质)增生,基膜增厚,血管壁纤维组织样增生硬化。灵芝孢子粉组生精小管层次比较清析,管腔规则,有大量精子生成,间质无增生,血管基膜增生硬化不明显。结论:灵芝孢子粉能减少自由基对睾丸组织的损伤,提高SDH活性,从而对糖尿病大鼠睾丸组织起保护作用。Objective： To investigate the changes of xanthine oxidase （XOD）, myeloperoxidase （MPO） and mitochondrial succinate dehydrogenase （SDH） in the testis and the protective effect of ganoderma lucidum spores on the testicular tissue of rats with non-insulin-dependent diabetes mellitus （NIDDM）. Methods ： Fifty male Wistar rats were divided randomly into a model, a ganoderma and a normal control group, the first two groups injected with 2% STZ （25 mg/kg） through the peritoneum, and the last one with half-and- half sodium citrate/citrate buffer solution. Two weeks after normal diet, glucose tolerance tests were performed and the rats with abnormal glucose tolerance in the model and ganoderma groups received high-fat and high-carbohydrare food, the latter given ganoderma lucium spores （250 mg/kg · d） in addition, both for 10 weeks and all rats fed alone. Glucose tolerance tests were repeated 1 day before the end of the experiment and the testes of the rats were harvested for the determination of XOD, MPO and SDH. Results ： SDH was significantly lower （ P 〈 0.05） while XOD and MPO significantly higher in the model group than in the ganoderma and control groups （P 〈0.05 ）. The model rats exhibited abnormal convoluted seminiferous tubules, indistinct parietal layers, decreased or abolished gonepoiesis, luminal peripheral fibrous tissue （ interstitial substance） accrementition, basal lamina thickening, and vessel wall fibrous tissue accrementition and sclerosis. Conlusion ： Ganoderma lucidum spores can protect the testis of diabetic rats by reducing free radical-induced damage to the testicular tissue and enhancing the activity of SDH.

关 键 词：灵芝孢子粉 糖尿病 睾丸 黄嘌呤氧化酶 髓过氧化物酶 琥珀酸脱氢酶 

分 类 号：R285.5[医药卫生—中药学]