启膈散及其拆方对人食管癌Eca109细胞裸鼠移植瘤血管生成的抑制作用  

Inhibitory effect of Qigesan and its separated formula on angiogenesis of esophageal carcinoma Eca109 cell-transplanted nude mouse tumor

作　　者：司富春[1] 

机构地区：[1]河南中医学院中医药分子生物学实验室,河南省郑州市450008

出　　处：《世界华人消化杂志》2008年第28期3139-3145,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30171160;No.30371716~~

摘　　要：目的:探讨启膈散及其拆方抑制肿瘤血管生成的作用和机制.方法:取对数生长期的人食管癌EC9706细胞,接种于裸鼠右胁皮下制备荷瘤裸鼠模型.自接种后第2天开始,每天用40倍成人剂量的启膈散全方及其活血和化痰两个拆方水煎液灌胃给药,连续60d.用免疫组化方法检测移植瘤中Fac-Ⅷ因子相关抗原标记的微血管密度(MVD)和VEGF的表达,Westernblot方法检测肿瘤组织EGFR、PDGFR、VEGF及PLC-γ1蛋白表达.结果:各用药组肿瘤MVD与模型组相比明显降低(36.43±4.16,40.29±2.8742.43±3.04vessels/mm2vs48.57±7.45vessels/mm2,P<0.05或0.01).各组肿瘤MVD值与VEGF表达量呈正相关(r=0.712,P=0.0005).各用药组肿瘤EGFR、PDGFR、VEGF、PLC-γ1蛋白表达与模型组相比明显降低.启膈散及其拆方抑制裸鼠移植瘤MVD及EGFR、PDGFR、VEGF、PLC-γ1蛋白表达的作用以全方组最好,活血组次之.结论:启膈散及其拆方能够抑制肿瘤血管生成,其作用机制与抑制EGFR、PDGFR、VEGF及PLC-γ1蛋白表达相关.AIM： To explore the effects and mechanism of Qigesan and its separated formula on tumor angiogenesis.METHODS： Exponentially growing Eca109 human esophageal carcinoma cells （1 × 10^6 cells/mouse） were injected subcutaneously into the flank of 6- week-old female BALB/c nude mice. On the next day after inoculation, the decoctions （40 folds of adult dosage） of Qigesan （W） and its separated formula （blood-activating group P and resolving phlegm group R） were administered by oral gavage once a day for 60 days. Microvessel density （MVD） and VEGF expression in the transplanted tumor was detected using immunohistochemtry, and the protein expressions of EGFR, PDGFR, VEGF and phospholipase C-γ1 （PLC-γ1） were determined using Western blot.RESULTS： Microvessel density （MVD） in the transplanted tumor of all treatment groups were markedly decreased compared with that in con-trol group （36.43 ± 4.16, 40.29 ± 2.87 42.43 ± 3.04 vessels/mm^2 vs 48.57 ± 7.45 vessels/mm^2, P 〈 0.05 or 0.01） MVD and VEGF had statistically positive correlation by SPASS13.0 （r = 0.712, P = 0.0005）. The protein expressions of EGFR, PDGFR, VEGF and phospholipase C-γ1 （PLC-γ1） were obviously decreased compared with that in control group. Inhibitory effects of Qigesan and its separated formula on MVD and those protein expressions were the best in group W and sec-ond in group P.CONCLUSION： Qigesan and its separated for-mula can inhibit tumor angiogenesis, which is related to the inhibition of protein expressions of EGFR, PDGFR, VEGF and PLC-γ1.

关 键 词：启膈散 食管癌细胞 荷瘤裸鼠 肿瘤血管生成 表皮生长因子受体 血小板衍生生长因子受体 血管内皮生长因子 磷脂酶C-Γ1 

分 类 号：R735.1[医药卫生—肿瘤] R737.110.5[医药卫生—临床医学]