狗牙根白化病植原体16S rDNA序列  

Association of phytoplasma with Bermuda grass white-leaf disease

作　　者：谭卫军[1] 陈勇[1] 张武[1] 韩承畴[1] 谭志远[1] 张巨明[1] 

机构地区：[1]华南农业大学农学院,广州510642

出　　处：《微生物学报》2008年第10期1393-1397,共5页Acta Microbiologica Sinica

基　　金：广东省攻关基金(2006B20301045;2007A020300009-5);国家自然科学基金(30671381)~~

摘　　要：【目的】狗牙根(草坪草的一种)白化病是狗牙根的一种重要病害,在全世界均有分布。为了确定中国大陆狗牙根白化病病原,进一步与世界其他地区的病原相比有无特异性。【方法】采用植原体16S rDNA PCR扩增、序列分析、Southern分子杂交等技术对狗牙根健康植株及白化病患病植株进行研究。【结果】仅白化病植株扩增获得1.3kb的特异片段,序列分析表明特异片段与Candidatus Phytoplasm Cynodontis有99%同源性。Southern杂交有特异条带,大小在预期范围之内。证实了在中国大陆发生的狗牙根健株型白化病病原含有植原体。【结论】大陆狗牙根白化病病原中含有植原体,为该病病原的进一步鉴定与防治奠定了基础。[Objective] Bermuda grass white leaf is an important disease on Bermuda grass all over the world. The aim of this research is to identify the pathogen which leads to Bermuda grass white leaf occurring on the Chinese mainland. [Methods] PCR amplification technique, sequence analysis and Southern hybridization were used. [Results] A 1.3 kb fragment was amplified by PCR phytoplasma universal primers and total DNA sample extracted from ill Bermuda grass as the amplified template. Sequence analysis of the amplified fragment indicated it clustered into Candidatus Phytoplasm Cynodontis. Southern hybridization analysis showed differential cingulums. [Conclusion] The pathogen of Bermuda grass white leaf on the Chinese mainland contains phytoplasma, which provides a scientific basis for further identification, prevention and control of the disease.

关 键 词：PCR 狗牙根 白化病 植原体 同源性 SOUTHERN杂交 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]