人胃癌与癌前病变组织的固有荧光特征  

Autofluorescence characteristics in human gastric cancer and precancerous tissues

作　　者：陈颖[1] 吴云林[1] 吴巍[1] 蔚青[2] 胡伟国[3] 郭强苏[4] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化内科,上海市200025 [2]上海交通大学医学院附属瑞金医院病理科,上海市200025 [3]上海交通大学医学院附属瑞金医院外科,上海市200025 [4]上海交通大学医学院,上海市200024

出　　处：《世界华人消化杂志》2008年第27期3055-3059,共5页World Chinese Journal of Digestology

摘　　要：目的:分析胃良恶性黏膜的固有荧光差异.方法:收集胃癌手术标本和胃低级别上皮内瘤变黏膜标本制成冰冻切片,在激光共聚焦显微镜下观察并测定荧光强度.结果:固有荧光主要位于胃壁黏膜层和黏膜下层.与正常胃黏膜相比,在胃癌和低级别上皮内瘤变组织中平均绿红荧光强度比较低(1.57±0.69,2.06±0.51vs3.75±1.41,P<0.01,0.05).同一患者的正常胃黏膜固有荧光强度明显高于胃腺癌胃黏膜(P<0.05),而与低级别上皮内瘤变胃黏膜之间无显著差异.结论:胃癌黏膜绿色固有荧光强度变化可作为恶性病变的标志.AIM： To analyze the autofluorescence differences between the benign and malignant gastric mucosa. METHODS： The specimens of gastric cancer and low-grade intraepithelial neoplasia were collect-ed to make frozen sections. The autofluorescence intensity was observed and measured by laser confocal microscopy.RESULTS： The autofluorescence was mainly located in gastric mucosa and submucosa. In comparison with that in nomal gastric mucosa, the green-red fluorescence intensity in gastric cancer and low-grade intraepithelial neoplasia decreased significantly （1.57 ± 0.69, 2.06 ± 0.51 vs 3.75 ± 1.41; P 〈 0.01, 0.05）. The fluorescence intensity of green wavelength in normal gastric mucosa was significantly higher than that in gastric cancer mucosa, while it was significantly different from that in low-grade intraepithelial neoplasia mucosa. CONCLUSION： The change of green fluores-cence in gastric cancer mucosa may be a marker of malignant lesion.

关 键 词：胃癌 激光共聚焦 固有荧光 

分 类 号：R735.2[医药卫生—肿瘤] R173[医药卫生—临床医学]