不同方法制备聚羟基脂肪酸酯支架材料及扫描电镜结构观察  

Preparation of polyhydroxyalkanoates scaffold and determination by scanning electron microscopy

作　　者：林月娟[1] 张泰星[2] 李晓涛[3] 

机构地区：[1]汕头大学分析测试中心,广东省汕头市515063 [2]汕头大学理学院生物系,广东省汕头市515063 [3]汕头大学多学科研究中心,广东省汕头市515063

出　　处：《中国组织工程研究与临床康复》2008年第36期7081-7084,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景：聚羟基脂肪酸酯材料是一类具有良好生物相容性和完全可降解性的生物塑料，但由于生产成本相对较高因而暂时限制了其普及应用。目的：拟采用溶剂浇铸法、热致相分离法、分子自组装法制作聚羟基脂肪酸酯支架材料，并利用扫描电镜对3种多孔材料的结构进行观察分析。设计、时间及地点：组织工程材料学体外观察，于2007—10／2008—03在汕头大学多学科研究中心完成。材料：聚3．羟基丁酸己酸酯由清华大学微生物实验室友情赠送，聚3-羟基丁酸酯为中国江苏省南天股份有限公司产品。方法：①将1．0g马来酸化的聚3-羟基丁酸己酸酯溶于50mL氯仿中，加热温度60℃持续1h，所得溶液倾倒在120mm培养皿中，室温下挥发1d，置冰干机48h，溶剂充分蒸发。同法以聚乳酸制备支架材料作为对照。②将0．5g聚3-羟基丁酸己酸酯溶于20mL 1，4-二氧六环中，加热至80℃并不断搅拌使材料充分溶解，所得澄清液倒进50mL敞口烧杯后，迅速转移至液氮（-196℃）中孵育30min，置冰干机48h，除去溶剂。同法以聚乳酸制备支架材料作为对照。③将1．0g聚3-羟基丁酸酯溶于50mL 60℃氯仿中，得到澄清液后，每10mL溶液中加入2．5mL二氧六环，所得混合液用普通超声仪超声20min，4℃冷冻20h使溶液变成凝胶，用水浸泡凝胶1d，去水后-80℃冷冻1h，再置于冰干机中48h。主要观察指标：3种样品的表面形貌和空隙度扫描电镜观察结果。结果：溶剂浇铸法制备的聚乳酸膜非常平滑，而厚度约200μm的马来酸化的聚3-羟基丁酸己酸酯膜比较粗糙，且膜表面具有很多螺纹结构。热致相分离法制备的聚3-羟基丁酸己酸酯多孔支架比聚乳酸支架具有更大的孔隙度，平均孔径约达到500μm。分子自组装法制备的聚3-羟基丁酸酯纳米纤维支架具有连续的三维网状结构，纤维平均直径为80—350nm�BACKGROUND： Polyhydroxyalkanoates （PHA） is a family of biopolymers with good biodegradability and biocompatibility, however, the wider application has been limited due to relatively high cost of its productions. OBJECTIVE： To fabricate different kinds of PHA bio-scaffolds through three different ways, including solution casting method, thermally induced phase separation and molecule self-assembling method, and to analyze these three kinds of porous scaffolds by scanning electron microscopy. DESIGN, TIME AND SETTING： In vitro observation of tissue engineering materials was performed in Multidisciplinary Research Center of Shantou University from October 2007 to March 2008. MATERIALS： Poly（3-hydroxybutyrate-co-3-hydroxyhexanoate） （PHBHHx） was kindly donated from the Microbiology Laboratory of Tsinghua University. Poly（R-3-hydroxyhutyrate） （PHB） and polylactic acid （PLA） were purchased from Nantian Co. Ltd., （Jiangsu, China）. METHODS： 1.0 g maleated PHBHHx was dissolved in 50 mL chloroform and heated for 60 minutes at 60 ℃. The solution was poured into 120 mm Petri dishes, which were then maintained at room temperature to completely evaporate the chloroform. The evaporation of solvent resulted in the formation of film. The PLA film was made by same way as control. 0.5 g PHBHHx was dissolved in 20 mL 1, 4-dioxane under vigorous agitation and heated at 80 ℃. The clear polymer solution was poured into 50 mL beaker which was fast-frozen in liquid nitrogen tank and then incubated for 30 minutes at 196 ℃. Solvent was removed by a freeze-drying process lasting 48 hours. The PLA scaffold was made by same way as control. 1.0 g PHB was dissolved in 50 mL chloroform at 60 ℃ to become a clear solution. 2.5 mL dioxane was added into the 50 mL beaker with each 10 mL PHB chloroform solution. The mixtures were sonicated for 20 minutes and then incubated at 4 ℃ about 20 hours to allow gel formations from the mixtures. Then the gels were immersed into water for one day. Subsequently, th

关 键 词：组织工程 生物支架 聚羟基脂肪酸酯 聚羟基丁酸酯 扫描电镜 

分 类 号：R318[医药卫生—生物医学工程]