植物油中角鲨烯的GC/MS分析  

Determination of squalene in vegetable oils by GC/MS

作　　者：廖杰[1] 赵玉兰[1] 李宁[1] 周国艮[1] 

机构地区：[1]解放军总医院医学实验测试中心,北京100853

出　　处：《现代仪器》2008年第5期36-37,共2页Modern Instruments

摘　　要：建立植物油中角鲨烯的GC/MS分析方法,植物油经KOH-甲醇法皂化和三氟化硼衍生,经HP-5MS毛细管柱分离,以二十四烷酸为内标,用GC/MS选择性离子监测对角鲨烯进行定量测定。方法在0.0625～2.00μg/mL范围内线性关系良好,相关系数r〉0.9998;最低检测限为7.8ng/mL。日内精密度为1.63%-2.89%,日间精密度为1.70%-3.22%。加标平均回收率为89.58%～94.12%。用所建立的方法测定6种植物油中角鲨烯含量。A method was developed for the determination of squalene in vegetable oils by gas chromatography-mass spectrometry（GC/MS） with selective ion monitoring. After saponification with potassium hy- droxide and deveriation using borontriifluoride, vegetable oils were separated on a HP-5MS column. Ligno- ceric acid（CH3（CH2）22COOH） was selected as the internal standard. The The linear range of the method was O.0625-2.00pg/mL （r=0.9998）. The detection limit of squalene was 7.8ng/mL. The within- day RSD were 1.63%-2.89% The between-day RSD were 1.70%-3.22%, The average recovery of squalene was 90.60%. The method has been successfully applied in the determination of squalene in 6 vegetable oils.

关 键 词：气相色谱/质谱法(GC/MS) 角鲨烯 植物油 

分 类 号：TQ658[化学工程—精细化工] TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]