两种方法提取甘草酸效率之比较  

Comparative Study on Two Extraction Methods of Glycyrrhizic Acid

作　　者：郑津辉[1] 王威[1] 卢莹[1] 

机构地区：[1]天津师范大学化生学院,天津300387

出　　处：《中国中医药信息杂志》2008年第10期46-48,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的比较水酸法和氨水法提取甘草酸含量的变化。方法利用正交试验4因素3水平选择最佳提取条件(包括加水量、提取次数、煮沸时间、粉碎时间、溶剂量、振荡时间、氨水浓度),用紫外分光光度法在252nm波长处测定甘草酸含量。结果每100g甘草粗粉用水酸法提取的最佳条件为:每次加水量2000mL,提取2次,煮沸5min,粉碎20s;氨水法最佳条件为:每次加0.4%氨水2000mL,振荡2h,提取2次。最终氨水法提取率为32%,水酸法提取率为26%。结论在最佳条件下,氨水法提取的甘草酸纯度和提取率明显高于水酸法。Objective To compare diffenrent amounts of the glycyrrhizic acid extracted by two methodsdecoct method and Ammonia-water method. Methods The best extraction conditions including amount of affusion water and solvent, extraction time, boiling time, crash time, vibrating time and concentration of ammonia were choosed by orthogonal design. Amounts of the glycyrrhizic acid were determined by ultraviolet spectrophotometry at 252 nm wavelengh. Result The best extraction conditions from decoct method were that amount of affusion wate was 200 mL, extract 2 times, boiling time was 5 rain, crash time was 20 s. The best extraction conditions from Ammonia-water method were that amount of 0.4% ammonia was 200 mL, vibrating time was 2 h, extract 2 times. The extraction rate of Ammonia-water method was 32%, and the extraction rate of decoct method was 26%. Conclusion For glycyrrhizic acid extraction and purity, Ammonia-water has superior efficient than decoct method at the best conditions.

关 键 词：甘草 甘草酸 水酸法 氨水法 正交试验 

分 类 号：R283.5[医药卫生—中药学]