高效液相色谱法测定抗纤丸中芍药苷、阿魏酸和黄芩苷的含量  

Determination of paeoniflorin,ferulic acid and baicalin in Kangxian pills by HPLC

作　　者：魏智勇[1] 王俊华[1] 边景[1] 毕丽萍[1] 刘芳[2] 周晶[2] 张庆伟[2] 

机构地区：[1]天津传染病医院,天津市肝病研究所,天津300192 [2]天津医科大学,天津300070

出　　处：《中国医院药学杂志》2008年第19期1631-1633,共3页Chinese Journal of Hospital Pharmacy

基　　金：天津市卫生局科技基金项目(编号:05KYZ89)

摘　　要：目的：建立同时测定抗纤丸中芍药苷、阿魏酸、黄芩苷3种有效成分的含量方法。方法：Diamonsil C18色谱柱，流动相甲醇-1％磷酸，梯度洗脱；二极管阵列检测器（DAD）；检测波长分别为芍药苷230nm，阿魏酸316nm，黄芩苷280nm，柱温35℃。结果：在3个波长下同时测定3种化合物的含量：芍药苷、阿魏酸、黄芩苷的线性范围分别为0．200．60μg，0．025～0．075μg，1．25～3．75μg；平均加样回收率分别为100．87％，100．31％，101．05％；RSD分别为1．03％，0．71％，1．88％。结论：该方法灵敏准确、重复性好，可用于本制剂的质量控制。OBJECTIVE To establish an HPLC-DAD method to determine the content of paeoniflorin, ferulic acid and baicalin in Kangxian pills. METHODS The sample was determined by RP HPLC-DAD with a Diamonsil C18 column, using methanol- 1% phosphoric acid solution as a mobile phase.detecting wavelength at 230 nm,316 nm ,280 nm and the column temperature being 35℃. RESULTS The three effective components could be detected by this method. A good linearity of paeoniflorin, ferulic acid and baicalin was in the range of 0. 20 - 0. 60μg, 0. 025 - 0. 075μg, 1.25 - 3. 75 μg; the average recoveries were 100. 87%, 100. 31%, 101.05%. CONCLUSION The method is simple, accurate and reproducible. It can be used to control the quality of Kangxian pills.

关 键 词：高效液相色谱-二极管阵列检测器 抗纤丸 芍药苷 阿魏酸 黄芩苷 

分 类 号：R927[医药卫生—药学]