Skp2、p27^kip1蛋白在结直肠癌中的表达及其意义  

Expression and clinical significance of Skp2 and p27 kipi protein in colorectal carcinoma

作　　者：黄志军[1] 朱世泽 郭启祥[1] 

机构地区：[1]福建医科大学附属第二医院肿瘤科,泉州362000 [2]福建泉州医学高等专科学校

出　　处：《中华实验外科杂志》2008年第10期1279-1281,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨Skp2蛋白在结直肠癌发生、发展中的作用及其与p27^kip1蛋白表达的关系。方法应用免疫组织化学法结合计算机图像分析检测结直肠癌（80例）、腺瘤（20例）、正常组织（20例）中Skp2蛋白和p27^kip1蛋白的表达。结果由正常结直肠黏膜、腺瘤到癌，Skp2阳性表达率为O％、55％、93．8％，呈上升趋势（P〈0．01）；p27^kip1阳性表达率为100％、95％、87．5％，呈下降趋势（P〈0．05）。结直肠癌中Skp2表达与p27^kip1表达呈负相关（r=-0．311，P〈0．01）。Skp2蛋白在癌组织中的表达与结喜肠癌的分化程度、淋巴转移有关（P〈0．05）。结论结直肠癌Skp2蛋白过表达与p27^kip1蛋白降解有关，Skp2蛋白过表达可能是结直肠癌发生、发展的原因之一。Objective To study the expression of Skp2 protein in coloreetal carcinoma and its relationship with the expression of p27^kip1 and explore its role in the tumorigenesis and development of colorectal carcinoma. Methods Immunohistochemical staining method and a computerized image analysis system were used to detect the expression of Skp2 and p27^kip1 in 80 colorectal carcinomas ,20 cotorectal adenomas and 20 normal colorectal tissues. Results The expressions rate of Skp2 protein was 0,55% and 93.8 % （ P 〈 0.01 ）, and that of p27^kip1 protein was 87.5 % ,95 % and 100% （ P 〈 0.05 ）, respectively in the normal mucosa, adenoma and colorectal cancer. A significant inverse relation was observed between the expression of Skp2 and of p27^kip1 （ r = - 0.311, P 〈 0.01, Pearson correlation）. The expression of Skp2 was significantly associated with the loss of tumor differentiation and lymph node metastasis （ P 〈 0.01 ）. Conclusion Skp2 over-expression, which may lead to degradation of p27^kip1, may be one of reasons in carcinogenes and progression of colorectal carcinoma.

关 键 词：结直肠肿瘤 Skp2蛋自 泛素 

分 类 号：R735.34[医药卫生—肿瘤]