前列腺增生与前列腺癌中KLK11／TMPRSSmRNA表达比值  

Ratio of TMPRSS/KLK11 mRNA in BHP and prostate cancer

作　　者：蔡岳斌[1] 毕学成[1] 何慧婵[1] 钟惟德[1] 

机构地区：[1]广州市第一人民医院泌尿外科,510180

出　　处：《中华实验外科杂志》2008年第10期1332-1333,共2页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目（04003650）;广州市科技计划资助项目（200323-E4053）;国家“863”高科技计划资助项目（2006AA02A245）

摘　　要：目的以实时荧光定量PCR技术测定前列腺增生（BPH）与前列腺癌（PCa）组织标本KLK11／TMPRSSmRNA比值，探讨KLK11／TMPRSS比值在前列腺癌诊断的特异性意义。方法通过实时荧光定量PCR对23例PCa、37例BPH及3例正常前列腺组织KLK11／TMPRSS的表达，儿较其在PCa与BPH中组织定量的差异。结果BPH与PCa组织KLK11／TMPRSSmRNA的定量表达值分别为2．264±0．460与5．905±0．780，差异有统计学意义（P〈0．05）。结论实时荧光RT-PER定量检测KLK11／TMPRSSmRNA为前列腺癌的诊断提供了可靠的辅助指标。Objective To study the KLKll/TMPRSS mRNA expression in BPH and prostate cancer （PCa） tissue cells,and explore the value of KLKll/TMPRSS mRNA expression level in the diagnosis of PCa. Methods A sensitive and real-time quantitative PCR assay was developed to compare the different expression of KLKll/TMPRSS mRNA in BPH, PCa and normal prostate tissue cells in 23 samples of PCa,37 samples of BHP and 3 samples of normal prostate tissues. Results The expression ratio of KLKll/TMPRSS mRNA in BPH and PCa prostate tissue cells to normal prostate tissue cells was 2. 264±0. 460 and 5. 905±0.780 respectively （ P 〈 0.05）. Conclusion The detection of KLK11/TMPRSS mRNA expression by QRT-PCR can afford reliable and helpable information for diagnosis of PCa.

关 键 词：前列腺癌 肿瘤学 基因表达 临床诊断 

分 类 号：R737.25[医药卫生—肿瘤] R73[医药卫生—临床医学]