22例轻链型多发性骨髓瘤实验室检查特征及轻链多聚体的鉴定  

Laboratory Character of 22 Patients with Light-Chain Multiple Myeloma and the Identification of Light-Chain Polimer

作　　者：李畅[1] 赵文静[1] 耿红莲[1] 仲人前[1] 孔宪涛[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科全军临床免疫中心,上海200003

出　　处：《现代检验医学杂志》2008年第5期57-59,共3页Journal of Modern Laboratory Medicine

摘　　要：目的分析22例轻链型多发性骨髓瘤的实验室检查特征,增强对轻链型多发性骨髓瘤的了解,并进一步鉴定轻链多聚体的形成情况。方法应用散射速率比浊法进行血尿轻链定量,应用血清蛋白电泳和免疫固定电泳进行M蛋白的鉴定,用SDS-PAGE电泳分析轻链多聚体的形成情况,并与IgG型多发性骨髓瘤进行比较。结果轻链型多发性骨髓瘤完整免疫球蛋白分子呈明显降低并低于IgG型多发性骨髓瘤,尿液中排出大量的免疫球蛋白游离轻链,β2-微球蛋白和肌酐值高于IgG型多发性骨髓瘤,尿液中可以鉴定出明显的多聚体。结论轻链型多发性骨髓瘤的肾损害更为严重,尿液中游离轻链的表现比血中M蛋白的表现更明显,且尿液中轻链多聚体的形成对轻链的定量结果有一定的影响。Objective To analyze the laboratory characters of 22 patients with light-chain multiple myeloma and to identify light-chain polimer in the urine of these patients. Methods All patients were analyzed by serum and urine protein electrophoresis and immunofixation electrophoresis, and quantified by rate nephelometricmethod for heavy chain and light chain. The light-chain polimer in urine were identified by SDS-PAGE electrophoresis. Results The heavy chains in light chain multiple myeloma patients were obviously lower than those in IgG multiple myeloma patients. Much light chains were found in the patients＇ urine and the β2-MG and creatinine were also higher. Light-chain polimer appeared in the urine of light-ehain multiple myeloma patients. Conclusion Nephridium damage is more serious in light-chain multiple myeloma than in the contrast, and light-chain multiple myeloma patients have more obvious changes in urine than in serun. The light-chain polimer will disturb the detection of urine light chain by rate nephelornetriemethod.

关 键 词：轻链 骨髓瘤 轻链多聚体 

分 类 号：R730.43[医药卫生—肿瘤]