量子点CdTe对RAW264.7细胞凋亡和脂质过氧化水平的影响  

Effect of quatum dots on proliferation and apoptosis in RAW264.7 cells

作　　者：殷海荣[1] 唐萌[1] 夏婷[1] 陆敏玉[1] 杨扬[1] 陶拓[1] 王永[2] 付德刚[2] 

机构地区：[1]东南大学公共卫生学院 [2]东南大学生物科学与医学工程学院

出　　处：《中国现代医学杂志》2008年第18期2593-2596,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金项目资助(No:30671782);国家重点基础研究发展计划973项目资助(No:2006CB705602);国家863计划项目资助(No:2004AA302G40)

摘　　要：目的探讨量子点CdTe对小鼠腹腔巨噬细胞RAW264.7凋亡和脂质过氧化水平的影响。方法用MTT法检测细胞增殖状况;MDA、NO含量及SOD活性表示脂质过氧化水平;Hoechst染色进行细胞形态学观察;流式细胞术及琼脂糖凝胶电泳检测细胞凋亡。结果量子点处理组细胞的增殖能力明显受到抑制,NO、MDA含量及SOD活性显著上升,细胞凋亡百分率明显增高,并能检测到清晰的"梯状条带",与阴性对照组比较差异有显著性(P<0.05)。结论量子点CdTe能引起RAW264.7细胞凋亡和脂质过氧化反应发生。[Objective] To clarify the effect of quatum dots on apoptosis and lipid peroxidation in RAW264.7 cells[Methods] MTT, flow cytometry （FCM） assays and DNA ladder assays were performed to assess the cellular proliferation and apoptosis respectively. The content of MDA and NO ,the activity of SOD were detected with kit, and make cell Hoechst straining to observe the morphologic changes of apoptosis. [Results] Compared with the control, QDs decreased the proliferative activity significantly, apoptotic cell increased remarkably and ＂DNA ladder ＂could be observed clearly. In the QDs group, the content of NO and MDA, activity of SOD were more than those in the control. [ Conclusion] QDs rusults in oxidative stress injury and induced RAW264.7 cells apoptosis.

关 键 词：量子点 巨噬细胞 脂质过氧化 细胞凋亡 细胞毒性 

分 类 号：R361.3[医药卫生—病理学]