环磷酰胺预处理对鼠脑缺血再灌注损伤炎症反应和自由基的影响  

Effect of cyclophosphamide on inflammatory reaction and free radicals of cerebral ischemia reperfusion injury in rats

作　　者：李浩[1] 刘开祥[1] 俸军林[1] 吴岚[1] 曾爱源[1] 李清华[1] 唐永刚[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541002

出　　处：《中国现代医学杂志》2008年第18期2605-2608,共4页China Journal of Modern Medicine

基　　金：广西自然科学基金项目(No:0542110)

摘　　要：目的研究环磷酰胺对缺血再灌注损伤大鼠额顶部皮质细胞间黏附分子-1(ICAM-1)表达、髓过氧化物酶(MPO)活性、超氧化物歧化酶(SOD)和丙二醛(MDA)含量的影响。方法雄性Wistar大鼠60只,随机分为假手术组、缺血再灌注组、环磷酰胺预处理组。缺血再灌注组和环磷酰胺预处理组均采用石蜡线栓法建立大脑中动脉局灶性脑缺血90min再灌注24h模型。环磷酰胺预处理组于缺血前5d给予环磷酰胺腹腔注射,剂量为12.6mg/kg,1次/d,共6次。采用免疫组化法观察额顶部皮质ICAM-1表达,细胞化学法检测MPO活性、SOD和MDA含量的变化,并进行2,3,5-三苯基氯化四氮唑(TTC)染色检测脑梗死体积。结果假手术组未见ICAM-1表达,缺血再灌注组ICAM-1表达明显增加,环磷酰胺预处理组ICAM-1表达较缺血再灌注组减少,差异具有显著性(P<0.05)。假手术组MPO活性呈较低水平,缺血再灌注组MPO活性明显增加,环磷酰胺预处理组MPO活性较缺血再灌注组降低,差异具有显著性(P<0.05)。缺血再灌注组MDA含量较假手术组增加,环磷酰胺预处理组MDA含量较缺血再灌注组降低;缺血再灌注组SOD含量较假手术组降低,环磷酰胺预处理组SOD含量较缺血再灌注组增加,差异均具有显著性(P<0.05)。环磷酰胺预处理组脑梗死体积较缺血再灌注组明显缩小(P<0.05)。结论环磷酰胺对缺血脑组织具有保护作用,对缺血再灌注脑损伤后炎症级联反应及氧化性损伤抑制可能是其发挥脑保护作用的机制之一。[Objective] To study the effect of eyclophosphamide on ICAM-1 ,the MPO activity and the content of SOD and MDA of the ischemic frontal and parietal cortex of cerebral ischemia reperfusion （I/R） injury in rats. [Methods] In this experiment,60 male wistar rats were randomly divided into 3 groups, which were sham operated group, I/R group and cyclophosphamide pretreament group. After 90 min the middle cerebral artery occlusion （MCAO） model following 24 h reperfusion durations was made by suture-occluded method in I/R group and cyclophosphamide pretreament group. Cyclophosphamide was injected into abdominal cavity 5 days before cerebral ischemia-reperfusion-injury in the cyclophosphamide pretreament group, once daily,six times. After MCAO following 24 h of reperfusion, we investigated the expression of ICAM-1 with using immunohistochemistry,the MPO activity and the content of SOD and MDA of the ischemic frontal and parietal cortex. TIC staining was used to calculate the cerebral infarct volume. [Results] （1）Expression of ICAM-1 was not observed in brain tissue of sham operated group rats.In I/R group, expression of ICAM-1 increased. Compared with I/R group, cyclophosphamide reduced expression of ICAM-I（P 〈0.05）. （2）MPO activity was lower in brain tissue of sham operated group . Compared with sham operated group, MPO activity was elevated in I/R group. Compared with I/R group, the increasing of MPO activity was completely inhibited after reperfusion in cyclophosphamide pretreament group （P 〈0.05）. （3）Compared with sham operated group, the content of MDA in I/R group was higher. Compared with I/R group, the content of MDA was lower in cyclophosphamide pretreament group. Compared with sham operated group, the content of SOD in I/R group was lower.Compared with I/R group, the content of SOD was higher in cyclophosphamide pretreament group（P 〈0.05）. （4）Compared with I/R group, cerebral infarction volume was decreased in cyclophosphamide pretreament group （P 〈0.05）. [Conclusi

关 键 词：再灌注损伤 细胞间黏附分子-1 髓过氧化物酶 超氧化物歧化酶 丙二醛 环磷酰胺 

分 类 号：R-332[医药卫生] R743.31