聚乙二醇胁迫下茶树咖啡碱合成酶基因的差异表达  

The Differential Expression of the Caffeine Synthase Gene in Tea (Camellia sinensis L.) Plant under Polyethylene Glycol Stress

作　　者：孙云[1] 郭春芳[2] 王秀英[1] 

机构地区：[1]福建农林大学园艺学院,福州350002 [2]福建教育学院,福州350025

出　　处：《中国农学通报》2008年第10期78-82,共5页Chinese Agricultural Science Bulletin

基　　金：福建省自然科学基金资助项目"茶树水分高效利用的分子生理基础研究"(B0510025);福建省教育厅科技计划资助项目(JA05333)

摘　　要：应用荧光定量PCR的方法对茶树咖啡碱合成酶基因在PEG胁迫下的表达情况进行分析。结果显示,与对照相比,PEG胁迫下咖啡碱合成酶基因表达量随着胁迫时间延长呈现出先升高后降低的变化特点,胁迫的12h内表达量明显上调,胁迫6h表达量最大,之后明显下降,相对表达量变化范围为0.13-10.97。The differential expression of the caffeine synthase gene in tea plant under polyethylene glycol（PEG ） stress was analyzed by fluorescence quantitative polymerase chain reaction. The results indicated that comparing with control, the caffeine synthase gene expression increased significantly in the 12 hours and reached the maximum level 6 hours after PEG stress, and then decreased. The caffeine synthase gene transcription level in tea leaves follows a high-low pattern under PEG stress. The relative expression ratios of the caffeine synthase gene ranged from 0.13 to 10.97.

关 键 词：茶树 聚乙二醇胁迫 咖啡碱合成酶 荧光定量PCR 

分 类 号：S571.1[农业科学—茶叶生产加工] Q786[农业科学—作物学]