铁在寒地早粳花药培养中的效应研究  

The Iron Effect Study of Early Jjaponica Anther Culture in Cold Region

作　　者：张兰民[1] 黄晓群[1] 潘国君[1] 王瑞英[1] 刘传雪[1] 张淑华[1] 关世武[1] 冯雅舒[1] 李大林[1] 

机构地区：[1]黑龙江省农业科学院水稻研究所,佳木斯154026

出　　处：《中国农学通报》2008年第10期197-200,共4页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"高产优质多抗水稻育种技术研究及新品种选育"的子课题"北方粳稻育种技术研究与新品种选育"的部分内容(2006BAD01A01-6)

摘　　要：铁是水稻花培培养基的重要成分。通过对7份寒地早粳组合F1(或品系)花药在不同Fe2+含量培养基上离体培养,发现培养基中的Fe2+的含量对诱导率、绿苗分化率影响较大。结果表明在N6培养基中将Fe2+提高到32-40mg/kg诱导率最大,高于N6培养基(Fe2+含量为5.6mg/kg)1.4倍以上。在此以内随着Fe2+含量的增加诱导率增加,大于此含量诱导率降低,呈单峰曲线分布。分化培养基中Fe2+含量增加到40mg/kg分化率显著降低。但在32-40mg/kgFe2+培养基上诱导出的愈伤组织在MS培养基上的绿苗分化率差异明显,85.7%材料可使绿苗生产率提高到7.8%左右。因此适当提高诱导培养基中Fe2+含量可提高寒地早粳的花培效率。Iron is an important component in anther culture medium of rice. In this study, through vitro culturing anthers of 7 early japonica combinations F1 of cool region （lines） in medium of different Fe2＋ content , we found that Fe2＋ content have greater impact in the induction rate and the rate of green plant on differentiation. The results showed that Fe2＋ will be increased to 32~40 mg/kg had largest induced rate in N6 medium, higher than the N6 medium （Fe2＋ content is 5.6mg/kg） 1.4 times more, in this within, with Fe2＋ content increase, the induced rate increase, more than this content, the induced rate induce, showed a single peak of distribution. When Fe2＋ content in differentiation medium increased to 40mg/kg, the differentiation rate was significantly lower. But the rate of green plant on the differentiation of callus was obvious differences in MS medium, which was induced in the medium of 32~40 mg/kg Fe2＋ content, about 85.7% of materials can improve the efficiency of anther culture to 7.8%. So, we consider that increasing the Fe2＋ content in induced medium can increase anther culture efficiency of early japonica in cold region.

关 键 词：寒地早粳 花培 培养基 铁的效应 

分 类 号：S511[农业科学—作物学]