桃褐腐病菌的拮抗细菌CE的发酵条件研究  

Fermentation Conditions for Antagonistic Bacterium CE against Monilinia fruticola

作　　者：赵晓燕[1] 武硕[1] 杨海清[1] 刘素花[1] 刘正坪[1] 

机构地区：[1]北京农学院植物科学技术系,北京102206

出　　处：《中国农学通报》2008年第10期413-418,共6页Chinese Agricultural Science Bulletin

基　　金：北京市教委资助项目"拮抗细菌对桃褐腐病菌的抑制机理研究"(KM200710020003);北京市农委资助项目"重要动植物病害绿色防治技术示范推广"(20060119);北京市属市管高等学校人才强教计划

摘　　要：为了确定桃褐腐病菌拮抗细菌CE的最有利的发酵液的组成和发酵条件,分别采用了正交试验和单因素试验的方法。结果表明:发酵液中的C、N源的组合、C、N源的含量、金属离子对拮抗细菌CE的生长量和抑菌活性都有不同的影响,用4.5%的玉米粉和0.15%的酵母浸膏配制的发酵液对拮抗细菌CE生长量和抑菌效果都相对较好,另外Fe3+的添加能同时促进拮抗细菌的生长量和抑菌效果。在研究的几个发酵条件中,拮抗细菌CE的生长量和抑菌效果在一定范围内成正相关,即随生长量增大抑菌效果增强,说明抑菌物质的产生与活菌量的多少有关,最适的发酵条件为30℃,pH7,140r/min,接种量为10%,发酵时间为60h。The composition of fermented broth and fermentation conditions were optimized by orthogonal and monofactorial experiment respectively. Combination and content of the C and N sources, as well as mental ions, had different effect on the growth and antagonistic effect of bacterium CE. The broth composed of 4.5% corn flour and 0.15% yeast extract was better for both growth and antagonistic effect of CE. Adding of Fe3＋ promoted both bacterial growth and antagonistic effect. The antagonistic effect of bacterium CE was correlation with bacterial growth under the studied conditions in a certain extent, that is, the antagonistic effect was increased with the growth of CE. The most preferable fermentation condition was 30℃, pH7, 140r/min, 10% inoculation, and fermented for 60h.

关 键 词：桃褐腐病菌 拮抗细菌 发酵条件 

分 类 号：S436.621.13[农业科学—农业昆虫与害虫防治]