SD大鼠吗啡依赖易感性差异与脑内5-羟色胺转运体和5-羟色胺1A受体mRNA表达的关系(英文)  

Association of different susceptibilities to morphine with the expression of 5-HTT and 5-HT1AR mRNA in brain regions of SD rats

作　　者：谌红献[1] 王瑛[2] 叶敏捷[2] 张瑞岭[2] 李昌琪[3] 郝伟[1] 

机构地区：[1]中南大学湘雅二医院精神卫生研究所,长沙410011 [2]新乡医学院第二附属医院 [3]中南大学湘雅医学院神经生物学教研室,长沙410011

出　　处：《中南大学学报（医学版）》2008年第9期776-783,共8页Journal of Central South University ：Medical Science

基　　金：the grants of National Natural Science Foundation(30500180);National Basic Program(973 Project)of China(2003 CB515403)

摘　　要：目的:探讨5-羟色胺转运体(5-HTT)和5-羟色胺1A受体(5-HT1AR)在吗啡精神依赖易感性差异形成中的可能作用机制。方法:使用条件性位置偏爱模型,将大鼠分为高、低偏爱组,采用原位杂交对成瘾密切相关的3个脑区:内侧前额叶皮质(mPFC)、中脑腹侧被盖区(VTA)和伏隔核(NAc)内5-HT转运体和5-HT1A受体mRNA的表达在依赖和戒断过程中的动态变化。结果:在大鼠依赖状态下,5-HT转运体mRNA的表达在mPFC,VTA,Nac 3个区域,高吗啡CPP组显著低于低吗啡CPP组,而5-HT1A受体mRNA的表达却相反,高吗啡CPP组显著高于低吗啡CPP组(P<0.05);在戒断状态时正好相反,5-HT转运体mRNA的表达在mPFC,VTA,Nac 3个区域,高吗啡CPP组显著高于低吗啡CPP组,而5-HT1A受体mRNA的表达却是高吗啡CPP组显著低于低吗啡CPP组(P<0.05)。结论:5-HT转运体和5-HT1A受体可能参与了大鼠吗啡易感性差异的形成。Objective To investigate the possible mechanism for the different CPP susceptibilities. Methods Using a conditioned place preference(CPP) model,rats were selected into high and low preference groups.Using in situ hybridization,we examined the mRNA expression of 5-hydroxytryptamine transporter(5-HTT) and 5-hydroxytryptamine 1A receptor(5-HT1AR) in 3 crucial regions in addiction,namely the ventral tegmental area(VTA),the nucleus accumbens(NAc),and the medial prefrontal cortex(mPFC),during the dependence and withdrawal. Results During dependence state,the expression of 5-HTT mRNA in each of the regions in the high preference group was significantly lower than that of the low preference group,while higher expression of 5-HT1AR mRNA in each of the regions in the high preference group than that of the low preference group was found(P<0.05).During withdrawal state,the expression of 5-HTT mRNA in each of the regions in high preference group was significantly higher than that of the low preference group,while lower expression of 5-HT1AR mRNA in each of the regions in the high preference group than that of the low prefe-rence group was found(P<0.05). Conclusion 5-HTT and 5-HT1AR may play a role in diffe-rences in susceptibility to morphine.

关 键 词：易感性 吗啡依赖 5-羟色胺转运体 5-羟色胺1A受体 条件性位置偏爱 

分 类 号：R749.61[医药卫生—神经病学与精神病学]