5-脂氧酶/半胱氨酰白三烯途径不参与大鼠C6胶质瘤细胞缺氧缺糖损伤  

5-Lipoxygenase/cysteinyl leukotriene pathway is not involved in injury of rat C6 glioma cells induced by oxygen-glucose deprivation

作　　者：黄雪琴[1] 黄晓佳[1] 张丽慧[1] 戚玲玲[1] 卢韵碧[1] 张纬萍[1] 魏尔清[1] 

机构地区：[1]浙江大学医学院药理学系,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2008年第5期456-462,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(30772561);浙江省自然科学基金(Y204210)

摘　　要：目的：观察缺氧缺糖（OGD）是否损伤大鼠C6胶质瘤细胞,以及5-脂氧酶（5-LOX）/半胱氨酰白三烯（CysLT）途经是否参与OGD损伤。方法：在OGD处理并恢复不同时间后,观察C6细胞活性变化,以及5-LOX抑制剂和CysLT受体拮抗剂的影响;以免疫细胞化学法观察5-LOX蛋白的细胞内分布;以RT-PCR法检测CysLT1和CysLT2受体mRNA表达;并观察白三烯D4（LTD4）对C6细胞的作用。结果：OGD4-8h并恢复24-72h后,可诱导C6细胞损伤;5-LOX抑制剂和CysLT受体拮抗剂对OGD损伤无明显作用。OGD未诱导5-LOX的核膜移位。C6细胞高表达CysLT2受体,但CysLT1受体表达很微弱,OGD不影响它们的表达。此外,LTD4对C6细胞没有明显作用。结论：OGD可诱导C6细胞损伤,但5-LOX/CysLT途径不参与OGD诱导的损伤。Objective： To determine whether oxygen-glucose deprivation （OGD） induces C6 cell injury,and whether 5-lipoxygenase （5-LOX）/cysteinyl leukotriene （CysLT） pathway is involved in OGD-induced injury. Methods： After OGD treatment and recovery for various durations,the viability of C6 cells was determined,and the effects of 5-LOX inhibitors and CysLT receptor antagonists were investigated. Intracellular distribution of 5-LOX protein was detected by immunocytochemistry,and the mRNA expressions of CysLT1 and CysLT2 receptors were detected by RT-PCR. The effect of leukotriene D4 （LTD4） on C6 cells was also investigated. Results： OGD for 4～8 h followed by recovery for 24～72 h significantly induced C6 cell injury. Neither 5-LOX inhibitors nor CysLT receptor antagonists inhibited OGD-induced injury. OGD did not induce 5-LOX translocation into the nuclear membrane. C6 cells highly expressed CysLT2 receptor,but the expression of CysLT1receptor was much weaker; the expression was not affected by OGD. In addition,LTD4did not affect C6 cells significantly. Conclusion： OGD can induce C6 cell injury,but 5-LOX/CysLT pathway might be not involved in OGD-induced injury.

关 键 词：神经胶质瘤 缺氧缺血 脑 白三烯类/分析 白三烯B4/分析 半胱氨酸/分析 白三烯拮抗剂 花生四烯酸盐5-脂氧合酶 细胞 培养的 

分 类 号：R739.41[医药卫生—肿瘤]