急性B淋巴细胞白血病动物模型的建立  

Establishment of animal model with B lineage acute leukemia in nude mice for evaluation of new therapeutic agents

作　　者：李丽霞[1] 汤永民[1] 顾伟忠[1] 汤宏峰[1] 钱柏芹[1] 沈红强[1] 罗春芳[1] 

机构地区：[1]浙江大学医学院附属儿童医院血液肿瘤科,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2008年第5期511-514,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(编号:30170391);浙江省自然科学基金重点项目(编号:Z205166);面上项目(编号:301570)

摘　　要：目的:建立急性B淋巴细胞系白血病动物模型,为药物进行体内靶向杀伤研究奠定基础。方法:裸鼠用环磷酰胺预处理后,尾静脉注射5×106个Nalm-6细胞,观察小鼠症状,记录发病时间,取其各个组织做病理检查,了解肿瘤细胞浸润情况。结果:小鼠注射肿瘤细胞(19.4±0.55)d后出现严重消瘦、脊柱侧弯和弓背症状,平均死亡时间(24.75±0.87)d;病理切片观察到小鼠骨髓、肝、脾、肾、脑膜、脑实质、卵巢、肺都有肿瘤细胞浸润,部分肝脾组织出现坏死。结论:采用裸鼠成功建立了急性B淋巴细胞系白血病的动物模型,为靶向治疗白血病研究奠定了基础。Objective： To establish an acute leukemia animal model for testing new therapeutic agents in vivo. Methods： Nude mice were intraperitoneally injected with 2 mg cyclophosphamide,24 h later 5×10^6 acute B-cell leukemia Nalm-6 cells was inoculated via the tail vein,then monitored daily. When animals were paralyzed or dying,the organs including the liver,spleen,lung,heart,kidney,brain,bone marrow,pancreas,testes were removed and fixed with formalin,examined by routine histopathology. Results： After Nalm-6 cells were inoculated the mean survival of mice were （19.4±0.55）d （n=6）. The paralysis of mice was followed by weight loss,bent spines,hogback,cachexia and death. Histopathological examination showed that the tumor cells infiltrated liver,spleen,kidney,lung,meninges,interior cerebrum,the liver and kidney were the most affected organs. Conclusion： B lineage acute leukemia animal model has been successfully established in the nude mice,which is suitable for testing new therapeutic agents.

关 键 词：白血病 B细胞 急性 疾病模型 动物 白血病 环磷酰胺/药理学 

分 类 号：R733.71[医药卫生—肿瘤]