褐藻糖胶对人乳腺癌MCF-7细胞的增殖抑制和凋亡诱导作用  

Effect of fucoidan on inhibition of proliferation and induction of apoptosis in human breast carcinoma cell line MCF-7

作　　者：张金玉[1] 葛银林[1] 张晓[1] 张春辉[1] 薛美兰[1] 

机构地区：[1]青岛大学医学院生物化学与分子生物学教研室,山东青岛266021

出　　处：《中国病理生理杂志》2008年第10期1901-1904,共4页Chinese Journal of Pathophysiology

基　　金：青岛市科技局科技计划资助项目(No.03-1-YN-14)

摘　　要：目的:研究褐藻糖胶对体外培养的人乳腺癌MCF-7细胞增殖及凋亡的影响,并探讨其凋亡机制。方法:不同浓度(100mg/L、300mg/L、500mg/L、1000mg/L)褐藻糖胶处理MCF-7细胞48h后,应用四甲基偶氮唑蓝(MTT)法检测细胞的增殖;Hoechst33258染色、琼脂糖凝胶电泳法观察细胞的凋亡的形态学及生化改变;逆转录聚合酶链反应(RT-PCR)和蛋白印迹分别测定细胞bcl-2和baxmRNA及其蛋白的表达。结果:不同实验浓度(100mg/L、300mg/L、500mg/L、1000mg/L)的褐藻糖胶均能抑制MCF-7细胞的增殖(P<0.01),抑制率呈浓度依赖性增大;褐藻糖胶诱导MCF-7细胞凋亡数增加,且可见明显的、凋亡特有的DNA梯形条带;在褐藻糖胶存在下,bcl-2mRNA和蛋白表达减少,而baxmRNA和蛋白表达增加,Bcl-2/Bax比值下降(P<0.05)。结论:褐藻糖胶可抑制MCF-7细胞增殖,且诱导其凋亡,其凋亡机制是下调Bcl-2和上调Bax蛋白的表达。AIM： To investigate the effects and related mechanism of fucoidan on the proliferation and apoptosis in breast carcinoma cell line MCF - 7. METHODS： MCF - 7 cells were treated with different concentrations of fucoidan （ 100 mg/L, 300 mg/L, 500 mg/L, 1 000 mg/L） for 48 h. Cell viability was measured by MTT assay. Apoptosis morphological and biochemical changes were detected by Hoechst 33258 staining and agarose gel electrophoresis. The expression of bcl - 2 and bax was examined by RT - PCR and Western blotting. RESULTS ： Fucoidan at different concentrations （ 100 mg/L, 300 mg/L, 500 mg/L, 1 000 mg/L ） effectively inhibited the proliferation of MCF - 7 cells （P 〈 0.01 ）. The inhibitory ratio and apoptosis rate increased in a concentration -dependent manner. Agarose gel electrophoresis of DNA revealed the characteristic ＂ladder＂ pattern of apoptosis. Fucoidan down -regulated the expression of bcl -2 and up - regulated bax in the levels of mRNA and protein. The ratio of Bcl - 2 to Bax decreased as the concentrations of fucoidan increased （P 〈0. 05）. CONCLUSION： Fucoidan inhibits the cell proliferation by inducing cell apoptosis, and the apoptosis is related to the down - regulation of anti - apoptotic protein Bcl - 2 and up - regulation of apoptotic protein Bax.

关 键 词：褐藻糖胶 MCF-7细胞 基因 BCL-2 基因 bax 细胞凋亡 

分 类 号：R931.77[医药卫生—生药学] R737.9[医药卫生—药学]